[Histonet] DIF on paraffin embedded tissue

[James Watson]

Interesting comments.  The use of this sample depends on if the antigen survives fixation and paraffin embedding.  About 45-55% of our work is done with immunofluorescence on FFPE slides.  we quench the autofluorescence with a CuSO4 treatment. 

James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel    858-332-4647 Fax   858-812-1915 jwatson at gnf.org

-----Original Message-----
From: Simmons, Christopher via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, November 25, 2015 8:10 AM
To: Rene J Buesa <rjbuesa at yahoo.com>
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] DIF on paraffin embedded tissue

Useless sample
Sorry

Sent from my iPhone

> On Nov 25, 2015, at 11:02 AM, Rene J Buesa via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> No matter WHO to tell you to do WHAT, for IF purposes, that FFPE 
> tissue is USELESS.René
> 
> 
>    On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> 
> We have a tissue sample that was processed and paraffin embedded.  We 
> URGENTLY need to recover the tissue and perform Immunofluorescence on 
> the sample.
> Does anyone have a procedure.  HELP
>   
>     madeathridge at pastnashville.com
> 
>   
> 
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet