[Histonet] processing cycle

[Gudrun Lang]

Hi Allison,
we doubled the times of the regular processing protocol beginning with
longer absolute ethanol, intermedium and paraffin. Our regular protocol
takes 13 hours and the fatty-protocol takes 17 hours. We start it at about 2
pm with endtime at 8 am. So breast tissue is mostly embedded at the end of
the bulk of cassettes.

This improved cutting really in a great manner.  The problem occurs, that
fixation time is rather short, when grossing of breast is done just shortly
before processing. So we want our pathologists to gross the breast from the
day before rather early in the morning. 

Gudrun

-----Ursprüngliche Nachricht-----
Von: Eck, Allison via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Gesendet: Dienstag, 17. November 2015 20:15
An: 'histonet at lists.utsouthwestern.edu'
Betreff: [Histonet] processing cycle

Does anyone have a processing cycle that is good for fatty tissues like
breast that they would be willing to share?  This will be used on a VIP5.

Thank you in advance

Allison
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