[Histonet] Histonet Digest, Vol 144, Issue 14

Nexgen Pathology nexgenpath at gmail.com
Mon Nov 16 09:48:13 CST 2015


Hi, im a budding histotechnician at Nexgen Pathology Limited in Trinidad
and Tobago.
What are your protocols with respect to taking levels for biopsies? How
many levels do you usually take per biopsy?
Email me personally if you can @Nicholas009 at gmail.com

Thanks Alot
Nicholas Seepersad

*Nexgen Pathology*
6 Eastern Main Rd.
San Juan
Trinidad & Tobago
Tel./Fax. (868) 674-7284
nexgenpathology.com

On Sat, Nov 14, 2015 at 2:00 PM, <histonet-request at lists.utsouthwestern.edu>
wrote:

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> Today's Topics:
>
>    1. Mohs sections (Angela Yepes)
>    2. Re: IHC on old slides (Hobbs, Carl)
>    3. Re: #ExtMail#  Slide and cassette printers (Ellenburg, Deborah)
>    4. Histology Lab Survey Questions (Va Paula Sicurello)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 13 Nov 2015 13:23:56 -0500
> From: Angela Yepes <angelapatriciay at gmail.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Mohs sections
> Message-ID: <9AB1BDCC-37FF-497D-A479-B394A8D5FAE8 at gmail.com>
> Content-Type: text/plain;       charset=us-ascii
>
>
>
> > From: Angela Yepes <angelapatriciay at gmail.com>
> > Date: November 12, 2015 at 6:05:57 PM EST
> > To: histonet at lists.utsouthwestern.edu
> > Subject: Mohs sections
> >
> >
> >> From: Angela Yepes <angelapatriciay at gmail.com>
> >> Date: November 12, 2015 at 4:15:50 PM EST
> >> To: histonet-request at lists.utsouthwestern.edu
> >> Subject: Mohs sections
> >> Hello everyone:
> >> While cutting Sections for Mohs surgery I noticed the tissue detaching
> from the O.C.T. Embedding medium  and subsequent rolling of the tissue.
> >> To correct the problem the chuck was rotated to allow cutting from the
> opposite side (epidermis side). This maneuver did not correct the problem.
> I tried to unroll the edges with stiff brush which seemed to correct the
> problem, but it did not. I only  found out the sections were still rolling
> up after the staining.
> >> The cryostat temperature was -23C and the sections thickness was -14
> microns
> >> Please see pictures.
> >> Please help
> >>
> >>
> >>
> >>
> >> Sent from my iPhone
> >>
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 13 Nov 2015 19:25:04 +0000
> From: "Hobbs, Carl" <carl.hobbs at kcl.ac.uk>
> To: histonet <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] IHC on old slides
> Message-ID:
>         <
> VI1PR03MB1407C1C22A150F0A81DC6F04C4110 at VI1PR03MB1407.eurprd03.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Amplification is the only way.
>
> Possibly, tyramide amplification will help.
>
> It will cost you to buy a kit...sure, make your own but....you have to
> weigh up pros/cons
>
>
> Sure....you will have to play with variables.
>
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 13 Nov 2015 19:42:29 +0000
> From: "Ellenburg, Deborah" <DEBORAH_ELLENBURG at bshsi.org>
> To: Mike Pence <mpence at grhs.net>
> Cc: "histonet at lists.utsouthwestern.edu"
>         <histonet at lists.utsouthwestern.edu>,
>         "histonet-bounces at lists.utsouthwestern.edu"
>         <histonet-bounces at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] #ExtMail#  Slide and cassette printers
> Message-ID: <66C85B18-BCE2-4F2D-9FF0-7136B605CE72 at bshsi.org>
> Content-Type: text/plain; charset="us-ascii"
>
> We have the ShurMark slide and cassette engraver that interfaces with
> CoPath.
>
> Sent from my iPhone
>
> > On Nov 12, 2015, at 10:50 AM, Mike Pence via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
> >
> > Okay, I am reaching out to the people that I know that has an answer to
> everything. What system are you using to print slides and cassettes from in
> your lab? Is it intergraded with your LIS and if so what LIS are you using?
> I am not looking for a system that prints to labels, I what it to print
> directly to the slide or cassette.
> >
> > Michael S. Pence | Supervisor of Laboratory Services
> > Great River Health Systems
> > 1221 S. Gear Ave. | West Burlington, IA 52655
> > Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557
> > mpence at grhs.net<mailto:mpence at grhs.net> | www.greatrivermedical.org<
> http://www.greatrivermedical.org>.
> > www.Facebook.com/GreatRiverHealthSystems<
> http://www.Facebook.com/GreatRiverHealthSystems> |
> www.Twitter/GreatRiverMed<http://www.Twitter/GreatRiverMed>
> >
> >
> > Information in this communication, including attachments, is
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> ------------------------------
>
> Message: 4
> Date: Sat, 14 Nov 2015 16:39:04 +0000 (UTC)
> From: Va Paula Sicurello <vapatpxs at yahoo.com>
> To: "histonet at lists.utsouthwestern.edu Histonet"
>         <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Histology Lab Survey Questions
> Message-ID:
>         <970743067.4496622.1447519144079.JavaMail.yahoo at mail.yahoo.com>
> Content-Type: text/plain; charset=UTF-8
>
> Hello Netters,
> Thank you for taking my survey. ?I will use your replies for my research
> project and I will also let everyone on the list know the results as well.
> >From what I've found, the surveys performed in the past asked similar
> questions but more at the institution level. ?I would like to get
> information at the individual level. ?There are 16 questions.
> Please reply to this email offline with your answers.
> Thank you for helping me. ?Let's hope I get an A! :-)
> Here we go:Questions aboutyou:
>
> 1.?????Education (highest achieved):
>
> a.??????High school
>
> b.?????Associates degree
>
> c.??????Bachelor?s degree
>
> d.?????Master?s degree
>
> e.??????PhD or MD
>
> 2.?????Certification:
>
> a.??????None
>
> b.?????HT
>
> c.??????HTL
>
> d.?????Other, please describe
>
> 3.?????How did you learn histology?
>
> a.??????On the job
>
> b.?????Histotechnology school
>
> 4.?????How many years of experience in histology?
>
> 5.?????How many blocks can you embed in an hour?
>
> 6.?????How many of the following can you produce in an 8
> hourday??????????????????????????? ????????????????????(You can calculate
> from howmany of each task you can perform in one hour:?20 blocks with one
> slide each per hour = 160 blocks and 160 slides)
>
> a.??????Blocks
>
> b.?????Slides
>
>
> Questions aboutyour place of work:
>
> 7.?????How many blocks does your lab embed a day?
>
> 8.?????How many staff does your lab have?
>
> a.??????Technical (HT, HTL, etc.)
>
> b.?????Non-technical (lab assistants, etc.)
>
> 9.?????How is IHC handled in your lab??
>
> a. Separate staff (if so how many?)?
>
> b. All staff perform
>
> 10.? Howmany IHC slides does your lab stain per day?
>
> 11.? Howmany of the following can your lab run?
>
> a.??????Antibodies (total number available for testing)
>
> b.?????Special Stains (total number available for testing)
>
> 12.? Doesyour lab use automation?? Yes or No
>
> Ifyes, please describe:
>
> 13.? Whoprepares the slides prior to release to the pathologist?
>
> 14.? Whofiles the blocks and slides?
>
> 15.? Whattype of institution do you work at?
>
> a.??????Hospital based Histology lab
>
> b.?????Clinic based Histology lab
>
> c.??????Independent lab (such as a referral lab)
>
> 16.? BonusQuestion:? Has your family stopped askingwhy you come home with
> blue or pink-orange fingers?
>
>
> ????????????????OPTIONAL: Please add your comments, suggestions,or
> thoughts.
> ?Sincerely,
> Paula
> Paula SicurelloHistotechnology SpecialistUC San Diego Health
>
>
> ------------------------------
>
> Subject: Digest Footer
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> ------------------------------
>
> End of Histonet Digest, Vol 144, Issue 14
> *****************************************
>


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