[Histonet] IHC hand staining

[Loralei Dewe]

I learned how to do all my IHC by hand from processing and embedding the
tissue to doing the cutting (manual microtome) and subsequent staining.  It
has really helped me in evaluating assays and in troubleshooting customers
problems!

Loralei Dewe
Chrysalis Innovations
On Nov 5, 2015 3:39 PM, "Carlos Defeo via Histonet" <
histonet at lists.utsouthwestern.edu> wrote:

> IHC staining for H.Pylori could be done using monoclonals from CellMarque
> or Biocare, with a simple protocol reccommended by the vendor,
> My regards,
> Carlos
> Histotochnologist
>
> ------ Mensaje original ------
> De: "Elaine allison Hoffman via Histonet" <
> histonet at lists.utsouthwestern.edu>
> Para: "Histonet List" <histonet at lists.utsouthwestern.edu>
> Enviado: 05/11/2015 13:05:38
> Asunto: [Histonet] IHC hand staining
>
> Greetings Histonetters,
>> I need some information on "hand" immuno-staining.  We are a small GI lab
>> doing Giemsa stains on H. Pylori right now, but the Lab Director wants me
>> to look into IHC hand staining for H.Pylori.  Anybody out there doing hand
>> staining and willing to give me a protocol to try? Or maybe point me in the
>> right direction to who could help me with that.  Any reliable IHC staining
>> kits recommended?  Thanks in advance for the help....
>> Elaine Hoffman
>> The Gastroenterology Clinic & Endoscopy Center, IncGI Pathology
>> Laboratory1622 E Market StreetWarren, Ohio   44483
>>
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