[Histonet] Aqueous Mounting Media

[Keyser Gerald T]

The slides are an important part of the medical record. Sure… I could assure that slide that are covered with media only are well treated while in my custody. But, those slides might see all sorts of abuse once they are outside of my care. They have to survive whatever pathologists, surgeons, residents, or fellows can throw at them. I feel better having a sturdy piece of glass over those sections.

Gerry

From: Caroline Miller [mailto:mills at 3scan.com]
Sent: Wednesday, November 04, 2015 7:05 PM
To: Keyser Gerald T
Subject: Re: [Histonet] Aqueous Mounting Media

Is there a reason you have to coverslip? I am presuming for storage. But I have found that the aqueous media alone is fine for imaging / viewing. If you are careful storing them they should be fine too.
I use this:
http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles&item=MMC0619
and I take a piece of parafilm, make sure the slide is wet, but not dripping (I take it out and give it a quick flick) put a drop of the aquaslip on the slide and use capillary action to pull it across the section, you have to be careful not to scratch the sample (make sure there are no nicks in the parafilm). It gives a nice flat surface, but it sounds like you are also getting some off-gassing from the product.
Oh Science!
Good luck!
mills

On Wed, Nov 4, 2015 at 6:08 AM, Keyser Gerald T via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>> wrote:
Currently my lab is using Aqua-mount (Lerner Laboratories). I'm looking at replacing it with a different Aqueous mounting media. The only stain we use it for is Oil Red O.

I've tried many different variations on its use and the results have been the same.

1)      The slide looks lovely the day that the coverslip is mounted and then dozens of bubbles form after. Sealing does not make a difference. Following company recommended procedure doesn't make a difference. Getting creative doesn't make a difference. Nothing seems to make a difference.

2)      We use a non-aqueous media surrounding the aqueous and post-coverslip the slide. This option is messy and cannot be re-coverslipped (for obvious reasons) if there is an air bubble. I'm sure that this wouldn't work at all with a more delicate stain.

Ok. I give up. Can anyone make a recommendation for a different aqueous mounting media?

Gerry
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--
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297