[Histonet] scanning with tape RE: In Defense of Tape

[Morken, Timothy]

FYI, We are manually coverslipping frozen slides with tape, due to the fact it dries very fast, and then scanning immediately for remote review. It is working very well. 


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-----Original Message-----
From: Terri Braud [mailto:tbraud at holyredeemer.com] 
Sent: Wednesday, May 13, 2015 10:58 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] In Defense of Tape

I've been using Tape coverslipping for over 20 years.  I've seen the problems with the tape lifting the section off of the slide, HOWEVER, there is a specific technical solution to keep this from occurring. Lifting occurs because of inadequate dehydration before Xylene clearing to coverslip.  Xylene is very forgiving of minute amounts of water carried over that might be microscopically undetectable, but Tape is not.  Any minute amount of water carried into the xylene before tape coverslipping will cause lifting.
I have 20 y/o slides coverslipped with tape with no issues.  I have heard that tape covered slides can't be scanned, but I've not confirmed this with a reliable source.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046


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