[Histonet] RE..... murine CD4, CD8 and CD68 for FFPE tissue

[Joseph Hardin]

Has anyone tried these Abs? If so, please send me your beautiful photos and protocol.

Joseph Hardin
Senior Research Specialist
UWCCC Experimental Pathology
WIMR I Rm. 4012
1111 Highland Av.
Madison, WI 53705
(608)262-1836


________________________________________
From: Gayle Callis <gayle.callis at bresnan.net>
Sent: Wednesday, May 13, 2015 12:34 PM
To: Histonet
Subject: [Histonet] RE.....  murine CD4, CD8 and CD68 for FFPE tissue

You wrote:



Is anyone of you familiar with the possibility of applying CD4, CD8 and CD68
antibodies on formalin fixed paraffin embedded liver of mice?



Thanks in advance.

Joost Bruijntjes



**************************************

Thankfully and at long last after years of frustration by many
................



eBioscience has both the CD8a and CD4 which is now available for FFPE murine
tissues.  Read the data sheets carefully as the CD4 indicated this antibody
was to be used on FFPE and not frozen sections.

            Anti-Mouse CD8a Purified Catalog Number: 14-0808 Also known as:
CD8 alpha, Ly-2, Ly-35, Lyt-2

            Anti-Mouse CD4 Purified Catalog Number: 14-9766 Also known as:
L3T4, Ly-4



Serotec has a rat anti mouse CD68 where the data sheet says can be used on
FFPE murine tissue.  This clone must be available from other companies as
well.



Gayle M. Callis

HTL/HT/MT(ASCP)



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