[Histonet] B-gal positive control

[koellingr at comcast.net]

Amos, hello.  Do you have a reference for this?  All my files talk about "endogenous" B-gal in kidney and pancreas and other organs (but then article talks about after lacZ transgenic manipulation) or demonstration of alpha-galactosidase in kidneys or in senescence associated or lysozomal storage diseases or differentiating light background staining from lacZ with pH tricks or pictures of islet cells staining with surrounding exocrine pancreas staining or just the journalistic form of hand-waving "data not shown".  Is beta-galactosidase readily expressed in completely normal kidney and where specifically? 
Thanks, Ray 
----- Original Message -----

From: "Amos Brooks" <amosbrooks at gmail.com> 
To: histonet at lists.utsouthwestern.edu 
Sent: Sunday, May 10, 2015 8:52:45 AM 
Subject: [Histonet]  B-gal positive control 

Hi, 
     Normal kidney should work fine for this. 
Amos 



On Fri, May 8, 2015 at 1:00 PM, <histonet-request at lists.utsouthwestern.edu> 
wrote: 

> Message: 9 
> Date: Fri, 8 May 2015 14:50:42 +0000 
> From: "Coffey, Anna (NIH/NCI) [C]" <anna.coffey at nih.gov> 
> To: "histonet at lists.utsouthwestern.edu" 
>         <histonet at lists.utsouthwestern.edu> 
> Subject: [Histonet] B-gal positive control 
> Message-ID: <5C3E10119A1B824FBE92B08279F74A91017995AF at msgb10.nih.gov> 
> Content-Type: text/plain; charset="us-ascii" 
> 
> Hello Histonet, 
> 
> Has anyone out there come across a good FFPE positive control for B-gal? 
> If so, please let me know! We would like to purchase a block or unstained 
> slides if at all possible. 
> 
> Thanks! 
> Anna 
> 
> Anna Coffey, MS, HTL(ASCP)CM 
> Histotechnologist 
> 
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