[Histonet] B-gal positive control

[koellingr at comcast.net]

Anna, 
Don't know if you are talking human or non-human control or if makes a difference and I don't want to get into that whole discussion again. But if a mouse control is OK, one of the cleanest and nicest systems I used  for B-gal was to get a transgenic mouse, easily obtainable with a Tie-2/lacZ promoter/reporter.  B-gal expressed only on vascular endothelium so your assay can easily be tweaked for strength and cleanliness of signal.  Have all the frozen/FFPE blocks you could ever need. 
Ray, Lake Forest Park, WA 

----- Original Message -----

From: "Anna Coffey (NIH/NCI) [C]" <anna.coffey at nih.gov> 
To: histonet at lists.utsouthwestern.edu 
Sent: Friday, May 8, 2015 7:50:42 AM 
Subject: [Histonet] B-gal positive control 

Hello Histonet, 

Has anyone out there come across a good FFPE positive control for B-gal? If so, please let me know! We would like to purchase a block or unstained slides if at all possible. 

Thanks! 
Anna 

Anna Coffey, MS, HTL(ASCP)CM 
Histotechnologist 
Center for Advanced Preclinical Research 
Frederick National Laboratory for Cancer Research 
Leidos Biomedical Research, Inc. 
Bld 539, 224 
Frederick, Maryland 21702 
anna.coffey at nih.gov 
301-846-1730 

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