[Histonet] Centrifuge for cytospins

[Piche, Jessica]

Thanks for sharing Joe! :)
Jessica

-----Original Message-----
From: Joe W. Walker, Jr. [mailto:joewalker at rrmc.org]
Sent: Tuesday, April 28, 2015 2:31 PM
To: Cooper, Brian; Piche, Jessica; histonet at lists.utsouthwestern.edu
Subject: RE: Centrifuge for cytospins

In all of my experiences with cytology preparations, the first step would be to centrifuge the specimen.  This concentrates the cells for the next step; cytocentrifuge, which is used for the presentation of the cells for evaluation.  Here is an excerpt from our procedure:

3.      Label a 50 ml centrifuge tube with the cytology accession label.
4.      Mix the specimen using a vortex mixer for 10-30 seconds.
5.      Pour contents of specimen from the original container into the 50 ml centrifuge tube and cap the tube.
6.      Centrifuge the specimen at 600g for 10 minutes (program 1) or 1200g for 5 minutes (program 2).
7.      Pour off the supernatant and re-suspend the sediment in 0.5 to 1ml of Cytospin (green) fluid, depending on the cell button size.

Evaluating the number of drops you might need for your cytocentrifuge can be performed by taking a crop from the sediment, placing it on a slide, placing a 24x50mm cover glass on the specimen and then observing the cellularity of the specimen.  I have document that illustrates this but I don't think the list serve will allow attachments.  You can email me privately for this document.

Joe W. Walker, Jr. MS, SCT(ASCP)CM
Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
P: 802.747.1790  F: 802.747.6525
Email joewalker at rrmc.org    www.rrmc.org

Our Vision:
To be the Best Community Healthcare System in New England

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-----Original Message-----
From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
Sent: Tuesday, April 28, 2015 12:10 PM
To: Piche, Jessica; histonet at lists.utsouthwestern.edu
Subject: [Histonet] RE: Centrifuge for cytospins

We make our BAL cytospins directly--no centrifuging first.

Brian

-----Original Message-----
From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Piche, Jessica
Sent: Tuesday, April 28, 2015 8:58 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Centrifuge for cytospins

Hi Everyone,

Just asking a quick question for our Cyto department. Are BAL slides centrifuged and then made in to cytospin slides or do you just make the cytospin slides with no centrifuging?

Thank you,

Jessica Piche, HT(ASCP)



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Thank You




CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital