[Histonet] Home made IHC breast markers: home made NBF vs Commercially prepared

[Anne Van Binsbergen]

Greetings esteemed Histonetters

We use commercially prepared NBF in pre-filled containers for collection and submission of all tissue. 

We use 'home-made' NBF ( Millonigs as per Freda Carson) in the holding tanks on the gross station and in all our tissue processors. 

We also strictly adhere to CAP regulations re fixation times for all breast tissue (6-72 hrs) - - our lower end cut-off is 8 hrs. 

Question: Could the use of 2 'different ' sources of NBF affect the staining of predictive markers?

Your expert opinion is highly appreciated

Regards from SandyLands
annieinarabia

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