[Histonet] Tissue transfer method:

[Whitaker, Bonnie]

I have used this for IHC, as well.  It's great stuff!  You can also use other, very viscous mounting medias, but the time it takes to dry/soak are longer than with Mount-Quick!

Thanks,
Bonnie

-----Original Message-----
From: Shirley A. Powell [mailto:POWELL_SA at mercer.edu] 
Sent: Friday, May 01, 2015 4:27 PM
To: James Watson; 'Jb'; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue transfer method:

With Mount Quick you can also decolorize the section and do a different stain on them.  I have even performed IHC on these transferred sections when needed.

Shirley Powell

-----Original Message-----
From: James Watson [mailto:JWatson at gnf.org] 
Sent: Friday, May 01, 2015 4:21 PM
To: 'Jb'; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue transfer method:

Slide Repair

1.	Soak coverslip off of slide in xylene.
2.	Piece slide back together.
3.	Apply Mount-Quick to slide, covering tissue. Allow to dry overnight.
4.	Place slide in cold water in refrigerator overnight.
5.	Carefully peal the Mount-Quick off the slide.  The tissue should come up with the Mount-Quick, if it does not stop pealing up the Mount-Quick and place back into the water overnight again.
6.	Carefully trim excess Mount-Quick from areas where tissue is not present. Leave at least ¼ inch of Mount-Quick around the tissue
7.	On a new slide that is wet with water, place the removed Mount-Quick and tissue in the center of the slide (make sure the tissue is next to the slide), removing as many air bubbles from under the Mount-Quick as possible.
8.	Cover the slide with a wet piece of filter paper then place a blank slide on top.  If you have multiple slides to repair then they can be stacked.  Apply weight to the top of this and let stand overnight.
9.	Soak the dry slide in Xylene until the Mount-Quick is removed and coverslip as usual.

James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel    858-332-4647 Fax   858-812-1915 jwatson at gnf.org

-----Original Message-----
From: Jb [mailto:craigak12 at gmail.com]
Sent: Friday, May 01, 2015 1:10 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Tissue transfer method:

Does anyone have a procedure that they can share on tissue transfer? One stained slide to another?

Thank you for your help.

Sincerely,

Craig

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