[Histonet] Bubble in MMA blocks

[Dorothy Hu]

Hi histonetter and specifically Gayle Callis,

I try to explain why we need to leave MMA embedded glass vials in water
bath during embedding to a research lab. But I need experts to have a
convencing explaination. The lab use vacuum desiccator (which has sealed
ring to keep vacuum) to leave the vials from 4 degree overnight, remove it
to room temperature for 3 hours, then move to 32 degree oven with the vials
in the vacuum dessiccator. They often found there are bubbles in the vial
after polymerization down. I knew they have change the way to do embedding
by remove the vial from dessiccator to close the cap and leave the vial in
water bath and space out to vent the heat generated during polymerization.
But how can I say the vacuum dessiccator.
Thanks everyone to explain.

Dorothy Hu