[Histonet] Question

[Goins, Tresa]

All that matters here is the final concentration of the reagent - it doesn't matter what stock you start with if you calculate the dilution.
Adding 1.25 ml to 1000 ml is diluted by a factor of .00125 so 10N x .00125 = .0125 N final concentration.
If using a 1 N stock solution, just add 10X the volume or 12.5 ml 1 N stock to 987.5 ml water.

Hope this helps,

Tresa

-----Original Message-----
From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Bernice Frederick
Sent: Thursday, April 30, 2015 1:35 PM
To: Histonet at lists.utsouthwestern.edu
Subject: [Histonet] Question

All,
I have a procedure here that call for and I quote "1.25 ml NaOH 10N in 1L of water." I know how to get 1 N, but how do I get 10. Having rarely hd the opportunity to make many Normal solutions ,my brain is not computing. Is it an error?
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick at northwestern.edu<mailto:b-frederick at northwestern.edu>

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