[Histonet] Re: Masson's Trichrome
Shelly Coker
sccrshlly <@t> yahoo.com
Wed Mar 11 22:07:10 CDT 2015
PTPM Acid is the bridge between the collagen and the aniline blue. It links the dye to the collagen. If you rinse the PTPM acid before putting the slide in the Aniline blue, you will not get the desired intensity of staining (of the collagen) that you are looking for.
Cheers and Have a great week!
> Here is a message from Justine...>
> From: Justine Lanzon [mailto:justinelanzon <@t> hotmail.com]
> <justinelanzon <@t> hotmail.com]>
> Sent: Thursday, March 05, 2015 5:36 AM
> To: lindamargraf <@t> gmail.com
> Subject: Masson's trichrome stain
>
>
> Hi,
>
> I am doing a write up on Masson's trichrome stain however I cannot
> answer these two questions:
>
> - Why are plastic forceps used instead of metal ones to hold the
> stained slide?
>
> - Why do we not rinse before Alinine blue?
>
> ?
>
> Can you please help me?
>
> ?
>
> Many Thanks,
>
> Justine Lanzon
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
We have literally about one hundred slides to re-slip for the this reason. Are there any suggestions for large numbers of slides to be re-coverslipped as this method would be too time consuming. We have used only glass for about nine years or so and it is much better. The old ones are the problem when someone needs "THAT" slide only.
Pam Marcum
UAMS
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N
Sent: Wednesday, March 11, 2015 10:43 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] RE: Old slides
Bernice,
Take the slide and dip it in xylene. Lay it on the film, pressing down firmly. As it adheres, then gently wipe the excess xylene off, and gently place it in a book or your procedure manual and leave it there for an hour or so.
Most of the bubbles will be gone, and the tissue will be saved.
The original problem is not enough xylene dispersed onto the slide. Adjust the flow being dispensed by the unit.
Sincerely,
Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center
713.563-3481
----------------------------------------------------------------------
Message: 1
Date: Mon, 9 Mar 2015 19:41:48 +0000
From: Bernice Frederick <b-frederick <@t> northwestern.edu>
Subject: [Histonet] Old slides.
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<eb9d7062461640e5ac22d213a4acc16f <@t> evcspmbx03.ads.northwestern.edu>
Content-Type: text/plain; charset="us-ascii"
Hi all,
We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there.
Thanks,
Bernice
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu<mailto:b-frederick <@t> northwestern.edu>
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Hello,
We received some liver tissue (Mouse) in 10% formalin (NFB). Then transferred to 70% ETOH. My question is that, Is it ok to transfer to 30% sucrose? So, frozen section can be perform. Please advise.
Thanks,
Bryan S.
-----Original Message-----
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Sent: Tuesday, March 10, 2015 10:06 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 136, Issue 12
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Today's Topics:
1. Old slides. (Bernice Frederick)
2. RE: Old slides. (Jason McGough)
3. RE: Mushrooms for GMS fungus control (Morken, Timothy)
4. Re: FW: Masson's trichrome stain (John Kiernan)
5. RE: Old slides. (John Kiernan)
6. soft for microwriter (thermo scientific, Lamb, Shandon)
(richard wild)
7. Re: Old slides. (b.curran.mcwilliam <@t> gmail.com)
8. Re: Old slides. (Rene J Buesa)
9. RE: Old slides. (Gowan,Christie C)
10. IHC / Morphometry Technician wanted in Shenandoah Valley
Virginia (Erin Sarricks)
----------------------------------------------------------------------
Message: 1
Date: Mon, 9 Mar 2015 19:41:48 +0000
From: Bernice Frederick <b-frederick <@t> northwestern.edu>
Subject: [Histonet] Old slides.
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<eb9d7062461640e5ac22d213a4acc16f <@t> evcspmbx03.ads.northwestern.edu>
Content-Type: text/plain; charset="us-ascii"
Hi all,
We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there.
Thanks,
Bernice
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu<mailto:b-frederick <@t> northwestern.edu>
------------------------------
Message: 2
Date: Mon, 9 Mar 2015 14:20:09 -0600
From: Jason McGough <jmcgough <@t> clinlab.com>
Subject: RE: [Histonet] Old slides.
To: Bernice Frederick <b-frederick <@t> northwestern.edu>,
histonet <@t> lists.utsouthwestern.edu <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <zarafa.54fe0079.5165.2525b65b03ddef5e <@t> mail.clinlab.com>
Content-Type: text/plain; charset=utf-8
Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method.
Jason McGough, HT(ASCP)
Operations Manager
Clinical Laboratory of the Black Hills
605-343-2267
jmcgough <@t> clinlab.com <mailto:jmcgough <@t> clinlab.com>
www.clinlab.com <http://www.clinlab.com>
-----Original message-----
> From:Bernice Frederick <b-frederick <@t> northwestern.edu
> <mailto:b-frederick <@t> northwestern.edu> >
> Sent: Monday, March 9, 2015 1:51 PM
> To: histonet <@t> lists.utsouthwestern.edu
> <mailto:histonet <@t> lists.utsouthwestern.edu>
> Subject: [Histonet] Old slides.
>
> Hi all,
> We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there.
> Thanks,
> Bernice
>
> Bernice Frederick HTL (ASCP)
> Senior Research Tech
> Pathology Core Facility
> Robert. H. Lurie Cancer Center
> Northwestern University
> 710 N Fairbanks Court
> Olson 8-421
> Chicago,IL 60611
> 312-503-3723
> b-frederick <@t> northwestern.edu <mailto:b-frederick <@t> northwestern.edu>
> <mailto:b-frederick <@t> northwestern.edu
> <mailto:b-frederick <@t> northwestern.edu> >
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> <mailto:Histonet <@t> lists.utsouthwestern.edu>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> <http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
>
>
------------------------------
Message: 3
Date: Mon, 9 Mar 2015 22:46:08 +0000
From: "Morken, Timothy" <Timothy.Morken <@t> ucsf.edu>
Subject: [Histonet] RE: Mushrooms for GMS fungus control
To: "koellingr <@t> comcast.net" <koellingr <@t> comcast.net>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<761E2B5697F795489C8710BCC72141FF367FBA31 <@t> ex07.net.ucsf.edu>
Content-Type: text/plain; charset="utf-8"
Try this article...
Acta Cytol. 2003 Nov-Dec;47(6):1043-4.
Alternative, cost-effective fungus-staining method for control slides in cytology and histopathology.
da Silva VD1.
Author information
Abstract
OBJECTIVE:
To develop a cost-effective, reliable and safe method of providing fungal control slides for routine use in pathology laboratories.
STUDY DESIGN:
A set of easily available, low-cost material was tested to obtain fungal colonies on substrate adequate for paraffin-embedded sections or smears.
RESULTS:
Such material as cheese is a simple, inexpensive and practical culture medium for silver-positive fungi. A batch of paraffin blocks can be prepared to maintain a stock of control material in the laboratory.
CONCLUSION:
It is useful to maintain fungal colonies to produce staining control specimens using small pieces of refrigerated cheese to easily produce silver-staining control specimens or smears embedded in paraffin, reducing the risk of accidental exposure to potentially infective pathogens in the laboratory. This method might also be a good alternative for conserving routine surgical specimens, considering the currently decreasing numbers of necropsy and large specimens, particularly from immunosuppressed and infected patients.
PMID: 14674076 [PubMed - indexed for MEDLINE]
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of koellingr <@t> comcast.net
Sent: Sunday, March 08, 2015 4:29 PM
To: Linda Prasad (SCHN)
Cc: Jeffrey Robinson; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Mushrooms for GMS fungus control
Apparently there are numerous interesting ways for fungus or bacteria controls to be had from orange peels to hamburger to slim Jim's to hot dogs to strawberries to ????.?? Sounds like fun to me.?? I'm curious, with the emphasis now on quality control in labs??run amok, has anyone passed a rigorous inspection actually showing these as your currently in-use controls??? A PI in research who??doesn't want??his paper rejected at peer review.?? A CAP inspector in clinical labs who is nit-picky reviewing staining controls but might be looking for a phase anything deficiency.?? The??dot-your-i's and cross-your-t's??FDA people who might or might not OK your drug in development.?? Really, just curious if anyone with a hammer over your head has said it is perfectly fine to use them.
Ray, Seattle, WA
----- Original Message -----
From: "Linda Prasad (SCHN)" <linda.prasad <@t> health.nsw.gov.au>
To: "Jeffrey Robinson" <JRobinson <@t> pathology-associates.com>, histonet <@t> lists.utsouthwestern.edu
Sent: Sunday, March 8, 2015 4:09:02 PM
Subject: [Histonet] RE: Mushrooms for GMS fungus control
I used strawberries for a fungal control. Worked really good.
Linda Prasad | Senior Scientist | Histopathology
t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad <@t> health.nsw.gov.au | w: www.schn.health.nsw.gov.au
Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia
???????Please consider the environment before printing this email.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson
Sent: Saturday, 7 March 2015 4:16 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mushrooms for GMS fungus control
How about mushrooms? ??Has anyone had any success using mushrooms as a GMS fungus control?
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA
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------------------------------
Message: 4
Date: Tue, 10 Mar 2015 00:31:56 -0500
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] FW: Masson's trichrome stain
To: Linda Margraf <lindamargraf <@t> gmail.com>,
histonet <@t> lists.utsouthwestern.edu
Cc: justinelanzon <@t> hotmail.com
Message-ID: <7380eaed48941.54fe3b7c <@t> uwo.ca>
Content-Type: text/plain; charset=iso-8859-1
The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters?
John Kiernan
= = =
On 08/03/15, Linda Margraf <lindamargraf <@t> gmail.com> wrote:
> Here is a message from Justine...
>
> From: Justine Lanzon [mailto:justinelanzon <@t> hotmail.com]
> <justinelanzon <@t> hotmail.com]>
> Sent: Thursday, March 05, 2015 5:36 AM
> To: lindamargraf <@t> gmail.com
> Subject: Masson's trichrome stain
>
>
> Hi,
>
> I am doing a write up on Masson's trichrome stain however I cannot
> answer these two questions:
>
> - Why are plastic forceps used instead of metal ones to hold the
> stained slide?
>
> - Why do we not rinse before Alinine blue?
>
> ?
>
> Can you please help me?
>
> ?
>
> Many Thanks,
>
> Justine Lanzon
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 5
Date: Tue, 10 Mar 2015 00:40:02 -0500
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: RE: [Histonet] Old slides.
To: Jason McGough <jmcgough <@t> clinlab.com>, Bernice Frederick
<b-frederick <@t> northwestern.edu>, histonet <@t> lists.utsouthwestern.edu,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <73e09aa74b442.54fe3d62 <@t> uwo.ca>
Content-Type: text/plain; charset=iso-8859-1
Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one.
John Kiernan
Anatomy & Cell Biology, UWO
London, Canada
= = =
On 09/03/15, Jason McGough <jmcgough <@t> clinlab.com> wrote:
> Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method.
>
>
>
> Jason McGough, HT(ASCP)
>
> Operations Manager
>
> Clinical Laboratory of the Black Hills
>
> 605-343-2267
>
> jmcgough <@t> clinlab.com <mailto:jmcgough <@t> clinlab.com
> <jmcgough <@t> clinlab.com>>
>
> www.clinlab.com <http://www.clinlab.com>
>
> ?
> ?
> -----Original message-----
> > From:Bernice Frederick <b-frederick <@t> northwestern.edu
> > <mailto:b-frederick <@t> northwestern.edu <b-frederick <@t> northwestern.edu>>
> > >
> > Sent: Monday, March 9, 2015 1:51 PM
> > To: histonet <@t> lists.utsouthwestern.edu
> > <mailto:histonet <@t> lists.utsouthwestern.edu
> > <histonet <@t> lists.utsouthwestern.edu>>
> > Subject: [Histonet] Old slides.
> >
> > Hi all,
> > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there.
> > Thanks,
> > Bernice
> >
> > Bernice Frederick HTL (ASCP)
> > Senior Research Tech
> > Pathology Core Facility
> > Robert. H. Lurie Cancer Center
> > Northwestern University
> > 710 N Fairbanks Court
> > Olson 8-421
> > Chicago,IL 60611
> > 312-503-3723
> > b-frederick <@t> northwestern.edu <mailto:b-frederick <@t> northwestern.edu
> > <b-frederick <@t> northwestern.edu>> <mailto:b-frederick <@t> northwestern.edu
> > <b-frederick <@t> northwestern.edu> <mailto:b-frederick <@t> northwestern.edu
> > <b-frederick <@t> northwestern.edu>> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > <mailto:Histonet <@t> lists.utsouthwestern.edu
> > <Histonet <@t> lists.utsouthwestern.edu>>
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > <http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
> >
> >
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 6
Date: Tue, 10 Mar 2015 10:05:11 +0100
From: richard wild <richard.wild <@t> wanadoo.fr>
Subject: [Histonet] soft for microwriter (thermo scientific, Lamb,
Shandon)
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <54FEB3C7.40306 <@t> wanadoo.fr>
Content-Type: text/plain; charset=utf-8; format=flowed
Hi
I am looking for labelling software (or advices) for the carousel microwriter (thermo scientific, Lamb, Shandon = same machine) (LAMB E22.01MWR) The machine is discontinuated.
I would like to use serial interface rs232 and barcode scanners.
Thanks for help.
Richard
------------------------------
Message: 7
Date: Tue, 10 Mar 2015 11:51:25 +0000
From: b.curran.mcwilliam <@t> gmail.com
Subject: Re: [Histonet] Old slides.
To: Bernice Frederick <b-frederick <@t> northwestern.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <9FA4B5FB-11BE-47C7-AA0C-6F8416B10487 <@t> gmail.com>
Content-Type: text/plain; charset=us-ascii
Hi
We re-Coverslipper a number of sections which had peeled off on the tape as the tape dried and curled. We cut off excess tape using scissors; placed a fresh coverslip flat; put a streak of mounting medium on he coverslip; use a forceps to orientate the tissue + margin of tape; number a slide, dip it in Xylene & place on a slope & bring on top of coverslip-section-mounting medium; turn the slide-section- coverslip to face coverslip up; leave horizontal to dry (eg overnight). Worth a try, doing one first.
Bernie,
St Vincent's,
Dublin,
Ireland
> On 9 Mar 2015, at 19:41, Bernice Frederick <b-frederick <@t> northwestern.edu> wrote:
>
> Hi all,
> We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there.
> Thanks,
> Bernice
>
> Bernice Frederick HTL (ASCP)
> Senior Research Tech
> Pathology Core Facility
> Robert. H. Lurie Cancer Center
> Northwestern University
> 710 N Fairbanks Court
> Olson 8-421
> Chicago,IL 60611
> 312-503-3723
> b-frederick <@t> northwestern.edu<mailto:b-frederick <@t> northwestern.edu>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Tue, 10 Mar 2015 13:43:35 +0000 (UTC)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Old slides.
To: John Kiernan <jkiernan <@t> uwo.ca>, Jason McGough
<jmcgough <@t> clinlab.com>, Bernice Frederick
<b-frederick <@t> northwestern.edu>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1897656965.1753669.1425995015431.JavaMail.yahoo <@t> mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8
To John
Probably the writer is referring to slides "mounted" with the Sakura film??"coverslip".I have done it many times and the FILM will??detach easily from the section.Had it been a glass coverslip attached with, for example, Permount, acetone would not have done anything.Ren?? J.??
On Tuesday, March 10, 2015 1:40 AM, John Kiernan <jkiernan <@t> uwo.ca> wrote:
Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one.
John Kiernan
Anatomy & Cell Biology, UWO
London, Canada
= = =
On 09/03/15, Jason McGough?? <jmcgough <@t> clinlab.com> wrote:
> Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method.
>
>
>
> Jason McGough, HT(ASCP)
>
> Operations Manager
>
> Clinical Laboratory of the Black Hills
>
> 605-343-2267
>
> jmcgough <@t> clinlab.com <mailto:jmcgough <@t> clinlab.com <jmcgough <@t> clinlab.com>>
>
> www.clinlab.com <http://www.clinlab.com>
>
> ??
> ??
> -----Original message-----
> > From:Bernice Frederick <b-frederick <@t> northwestern.edu <mailto:b-frederick <@t> northwestern.edu <b-frederick <@t> northwestern.edu>> >
> > Sent: Monday, March 9, 2015 1:51 PM
> > To: histonet <@t> lists.utsouthwestern.edu <mailto:histonet <@t> lists.utsouthwestern.edu <histonet <@t> lists.utsouthwestern.edu>>
> > Subject: [Histonet] Old slides.
> >
> > Hi all,
> > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there.
> > Thanks,
> > Bernice
> >
> > Bernice Frederick HTL (ASCP)
> > Senior Research Tech
> > Pathology Core Facility
> > Robert. H. Lurie Cancer Center
> > Northwestern University
> > 710 N Fairbanks Court
> > Olson 8-421
> > Chicago,IL 60611
> > 312-503-3723
> > b-frederick <@t> northwestern.edu <mailto:b-frederick <@t> northwestern.edu <b-frederick <@t> northwestern.edu>> <mailto:b-frederick <@t> northwestern.edu <b-frederick <@t> northwestern.edu> <mailto:b-frederick <@t> northwestern.edu <b-frederick <@t> northwestern.edu>> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu <mailto:Histonet <@t> lists.utsouthwestern.edu <Histonet <@t> lists.utsouthwestern.edu>>
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet <http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
> >
> >
>
>
> _______________________________________________
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Message: 9
Date: Mon, 9 Mar 2015 20:01:10 +0000
From: "Gowan,Christie C" <christiecgowan <@t> dermatology.med.ufl.edu>
Subject: [Histonet] RE: Old slides.
To: "'Bernice Frederick'" <b-frederick <@t> northwestern.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <de45f62de02d4c3389de0af3f45e191a <@t> AHC-EXCH03.ad.ufl.edu>
Content-Type: text/plain; charset="us-ascii"
Hi Bernice,
I have found that if you flood the slide with mounting media (don't use xylene) flip the slide over onto an absorbent lab wipe and put a heavy weight with even pressure and leave for a few hours. If the slide sticks to the wipe just put a few drops of xylene to clean up the slide. You may still have some tiny bubbles but it is much better than the alternative. Good luck.
Christie Gowan
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bernice Frederick
Sent: Monday, March 09, 2015 3:42 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Old slides.
Hi all,
We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there.
Thanks,
Bernice
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu<mailto:b-frederick <@t> northwestern.edu>
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Message: 10
Date: Tue, 10 Mar 2015 12:12:05 -0400
From: Erin Sarricks <esarricks <@t> gmail.com>
Subject: [Histonet] IHC / Morphometry Technician wanted in Shenandoah
Valley Virginia
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAPapK_1E+KJTXn9pLbWgLsVrd98Fdi1=ULpmhsbkSSdb8nrvQA <@t> mail.gmail.com>
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Histology Laboratory located in the Shenandoah Valley of Virginia is
looking to add to it's team. In this position, you will need a working
knowledge of IHC theory and practical IHC experience. The best candidate
for the position will oversee immunohistochemical staining as well as
perform other histology functions including trimming of specimens, paraffin
embedding, microtomy and microscopic QC of slides. Experience with
morphometry is preferable.
Desirable candidates will possess the following:
- HT (ASCP) or QIHC registration preferred
- 4 years of Histology experience
- 1+ years of immunohistochemistry and/or immunofluorescence experience
- Keeps abreast with company's current policies and immunohistochemistry
technical updates and procedures
- Must be able to work independently and in a team environment
Full time employment benefits include subsidized medical and dental
insurance, vacation, holiday pay, and 401k after 1 year of employment.
Compensation is commensurate with experience.
If you are interested in this position, please respond to this post with
your resume and cover letter.
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End of Histonet Digest, Vol 136, Issue 12
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