[Histonet] Phosphotungstic acid

[John Kiernan]

PTA can be used on tiny specimens to enhance electron-opacity. For light microscopy, PTA is used in the trichrome methods to enhance differential staining of collagen and cytoplasm, especially in paraffin sections. These uses of PTA are not the same as "soak formalin fixed soft tissue for a few hours before it gets processed". What are you hoping to achieve?

John Kiernan
London, Canada
= = =
On 25/03/15, "Cecilia A."  <cecystan76 <@t> gmail.com> wrote:
> Hello Histonetters,
> 
> I was wondering if anyone might be able to give some input regarding the
> use of Phosphotungstic acid in which we want to soak formalin fixed soft
> tissue for a few hours before it gets processed. Will subjecting it to PTA
> before processing affect its viability for further histology work (ie, H&E
> staining and IHC)?
> 
> Thank you very much!
> C.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
>