[Histonet] measuring cells

[Barry Rittman]

I apologize for misplacing the original posting about this.

 Measuring the number of cells in sections is very tricky and varies
considerably with the thickness of the section and the size of the cells.
In general the thicker the section the more accurate the count as there are
more complete cells rather than just profiles. The problem is that in order
to get a really accurate count you need sections that are so thick that
resolution suffers. Abercrombie in 1946 published papers that took into
account the size of cells and the thickness of the sections and depending
on these applied a correction factor that improved  the accuracy of the
count. His papers are worth reading. The link is below for those who are
interested.


http://www.nervenet.org/papers/Abercrombie46.html

Of course an interesting approach would be to cut several sections,
separate the cells and then use a Coulter counter... like this is going to
happen!!

Barry