[Histonet] Acetone fixing and tissue damage

[Lewis, Patrick]

Hi Everyone.

When I fix my cryosections  in acetone,  I am using HPLC grade 99.9% for 10 minutes at -20C.

Would the Histology grade 99.5% be less damaging to them?

Higher H20 content, i.e. less than 99.5% apparently is also very bad.

With the HPLC grade I often get tissue damage, the tissue also floats off the slide causing a stringy effect.


Fixing with 4% p-formaldehyde or 100% Methanol, prevented the antibody from recognizing the Nuclear Antigens.

Looking for advice,

Patrick.
Patrick Lewis
Research Associate II Bench
Seattle Childrens Research Institute
206-884-1115

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