[Histonet] Old slides.

[Rene J Buesa]

To John
Probably the writer is referring to slides "mounted" with the Sakura film "coverslip".I have done it many times and the FILM will detach easily from the section.Had it been a glass coverslip attached with, for example, Permount, acetone would not have done anything.René J.  

     On Tuesday, March 10, 2015 1:40 AM, John Kiernan <jkiernan <@t> uwo.ca> wrote:
   

 Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one.
John Kiernan
Anatomy & Cell Biology, UWO
London, Canada
= = =
On 09/03/15, Jason McGough  <jmcgough <@t> clinlab.com> wrote:
> Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method.
> 
> 
> 
> Jason McGough, HT(ASCP)
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> -----Original message-----
> > From:Bernice Frederick <b-frederick <@t> northwestern.edu <mailto:b-frederick <@t> northwestern.edu <b-frederick <@t> northwestern.edu>> >
> > Sent: Monday, March 9, 2015 1:51 PM
> > To: histonet <@t> lists.utsouthwestern.edu <mailto:histonet <@t> lists.utsouthwestern.edu <histonet <@t> lists.utsouthwestern.edu>> 
> > Subject: [Histonet] Old slides.
> > 
> > Hi all,
> > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there.
> > Thanks,
> > Bernice
> > 
> > Bernice Frederick HTL (ASCP)
> > Senior Research Tech
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