[Histonet] RE: Ventana Ultra CD10

[Jeffrey Robinson]

Hi Beth Ann-  I have also had problems with the Ventana CD10 after years of using it successfully.  I don't know if they changed something or what but it just dropped off all of a sudden.  I worked with one of their applications specialists on it for some time (different lot #'s, protocol changes, etc.) but was still unsuccessful in getting the problem resolved.  I also have a Leica Bond on which I also run CD10.  I ended up buying the Leica CD10 and putting it in a prep kit on the Ultra.  I have to use the amplifier with it and the protocol is a little long (3 1/2 hours I think) but it works OK.  I also have a Ventana Benchmark XT but I was unsuccessful in getting the Leica CD10 optimized on that instrument (I use the iView detection kit on that so I think that is the main problem).  I had a similar problem with the Ventana CD30 and had to switch to the CellMarque CD30 in that case but I was able to get that one optimized and validated on both the Ultra and the XT.

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of O'neil, Beth
Sent: Thursday, February 26, 2015 1:22 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ventana Ultra CD10

We are in the last stages of contract negotiations for purchasing two Ventana Benchmark Ultras.  During this time period we have been optimizing our current inventory of approximately 110 antibodies and validating before our current instrumentation is removed within the next two weeks.  My Ventana application specialist is unable to successfully optimize CD10 (Ventana clone SP67) on the Ventana Benchmark Ultra.  He had me send slides to their applications/troubleshooting lab but they told us they won't start working on it until next week and then it would take about a week for them to try and optimize it.  We are in an urgent rush to get this antibody optimized and validated within the next two weeks since it is heavily requested by our Hemepaths.  Would anyone be willing to share their Ultra protocols with me?  Has anyone had similar experiences with Ventana being unsuccessful and having to send their slides to their applications lab for work up?  Thank you for your help.

Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
oneilb <@t> wvuhealthcare.com<mailto:oneilb <@t> wvuhealthcare.com>
Histology Supervisor, Technical Specialist
Lab:  304 - 293 - 6014
Office:  304 - 293 - 7629



-----------------------------------------

Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information.  Any unauthorized review, use, disclosure or distribution is prohibited.  If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you.