[Histonet] Masson trichrome and H and E

[Wineman, Terra]

I use the kit from Sigma-Aldrich (HT15 = $300).


Terra Wineman, HTL (ASCP)CM
Research Biologist, Nutritional Physiology
636-926-7476 phone
terra.wineman <@t> novusint.com




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Tuesday, March 03, 2015 9:46 AM
To: Emily Brown; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Masson trichrome and H and E

Medical Chemical Corporation has a trichrome kit with 4 oz reagents for around $60.00.

Laurie Colbert, HT (ASCP)


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Emily Brown
Sent: Tuesday, March 03, 2015 4:39 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Masson trichrome and H and E

Hello!

I am doing Masson trichrome manually (not with a machine) and I just found out the kit is $800!! I was thinking of buying the ingredients separately, but why the hell is it so expensive???
Also, the people I was going to borrow their reagents from said their aniline blue is not very good and I wanted to replace it.  I only need about 250ml, what brand do you guys prefer?
l know I can google this, but I want to know what you guys like and what works best.  This is for mouse kidney paraffin sections, 4 to 5 microns.
Another question, I did H and E and there is no eosin staining.  I think the reagents are pretty old, so I thought that might be a problem.  Also because my lab is cheap, they were reusing the xylenes and EtOH for both rehydrating and rehydrating.  I told my boss this is probably not a good idea as the end steps will have stain in them.  And I also think this is why it didn't work! The EtOH is also really old so who know is the 100% is actually even close to 100% any more.  I'm buying new reagents, but if you guys think anything else would help, let me know.

Also, shoutout to Ann, I know you're reading these!! Join the list!!

Emily


"By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward."

-Chuck Palahniuk, "Haunted"
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