[Histonet] Masson trichrome and H and E

[Emily Brown]

Hello!

I am doing Masson trichrome manually (not with a machine) and I just found
out the kit is $800!! I was thinking of buying the ingredients separately,
but why the hell is it so expensive???
Also, the people I was going to borrow their reagents from said their
aniline blue is not very good and I wanted to replace it.  I only need
about 250ml, what brand do you guys prefer?
l know I can google this, but I want to know what you guys like and what
works best.  This is for mouse kidney paraffin sections, 4 to 5 microns.
Another question, I did H and E and there is no eosin staining.  I think
the reagents are pretty old, so I thought that might be a problem.  Also
because my lab is cheap, they were reusing the xylenes and EtOH for both
rehydrating and rehydrating.  I told my boss this is probably not a good
idea as the end steps will have stain in them.  And I also think this is
why it didn't work! The EtOH is also really old so who know is the 100% is
actually even close to 100% any more.  I'm buying new reagents, but if you
guys think anything else would help, let me know.

Also, shoutout to Ann, I know you're reading these!! Join the list!!

Emily


"By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward."

-Chuck Palahniuk, "Haunted"