[Histonet] Trichrome troubleshooting

Suzanne Martin smartin at lcpath.com
Tue Jun 30 13:37:10 CDT 2015


Hi all,

We are having trouble troubleshooting our trichrome. It is too blue. We are using Leica's kit with the Weigerts iron with Gills. Most of the small bowel controls have seen improvement but patient tissue is not... strange. 

We have tried lessening the time in Gills, adding time for the last acid step, even lessening time and adding time in the Weigerts. 


Thoughts?

Thank you.

Suzanne HT






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