[Histonet] Brain Tissue Processing

[Simmons, Christopher]

Autopsy Program 13h 36m						
						
Step	Reagent	Time	Temp.	Vac	Stir	Drip Time
1	Fixative	30	37	Cycle	Medium	10
2	Fixative	30	37	Cycle	Medium	10
3	Dehydrant	60	37	Cycle	Medium	10
4	Dehydrant	60	37	Cycle	Medium	10
5	Dehydrant	60	37	Cycle	Medium	10
6	Dehydrant	60	37	Cycle	Medium	10
7	Dehydrant	90	37	Cycle	Medium	10
8	Dehydrant	90	37	Cycle	Medium	10
9	Xylene		45	37	Cycle	Medium	10
10	Xylene		60	37	Cycle	Medium	10
11	Xylene		60	37	Cycle	Medium	10
12	Wax		40	60	Cycle	Medium	10
13	Wax		60	60	Cycle	Medium	10
14	Wax		60	60	Cycle	Medium	30

Works PERFECTLY every time!

Chris Simmons B.S., A.S., HTL(ASCP)
Supervisor, UPP Dermatopathology
412.864.3880 office
412.612.0881 cell


-----Original Message-----
From: Stoll, Kathryn [mailto:kstoll at mcw.edu] 
Sent: Monday, June 29, 2015 10:15 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Brain Tissue Processing

Hello,
I am looking for help with tissue processing of brain.  The pieces are large, they fill a super cassette.  Does anyone have a protocol they could share.
Thanks in advance.

Kathryn Stoll
Supervisor Histology
Clinical and Translational Research Core Lab Medical College of Wisconsin
9200 W. Wisconsin Ave Room 1176
Milwaukee WI 53226
Phone: 414-805-1525
Fax: 414-805-1528

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