[Histonet] IHC turnaround time

Sebree Linda A LSebree at uwhealth.org
Fri Jun 19 16:47:58 CDT 2015

We have 2 FTEs in IHC; myself as one of two people that started the lab 20+ years ago and 2 histotechs that rotate through on a weekly basis.  Our monthly totals currently run between 2400 and 3300.  Our TAT policy is "in by noon, out by 5pm" except for dual and triple stains (need to be on an instrument by 11:00 am) and EBER (needs to be on by ~ 9:00 am) and HER2 dual ISH (only run overnight).  We have 4 Roche (Ventana) Ultras that hold 30 slides each and are continuous access.  All instruments are used every day and at least one (usually 2 or 3) in use overnight.


Linda A. Sebree 
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory, Rm A4/204-3224 
600 Highland Ave. 
Madison, WI 53792 
FAX: (608)262-7174 

-----Original Message-----
From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] 
Sent: Friday, June 19, 2015 10:21 AM
To: Olszewski, Dawn; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC turnaround time

I direct a busy and very efficient (I think) IHC lab staffed with 2.875 FTEs who spend most of their time doing IHC, but they also help Histology embed and cut paraffin blocks when needed (which is most days).  For 2015 we are averaging 4,022 IHC patient test slides and 1,650 IHC control slides per month.  Our IHC lab is staffed from 4:30 AM to 6 PM (or later depending on workload).  We have 5 Leica Bond Max platforms; however, we still do some IHCs on the bench.  We usually do 2-3 runs per day and an overnight run (except on Fridays) as well.


Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-----Original Message-----
From: Olszewski, Dawn [mailto:Dawn.Olszewski at SGMC.ORG]
Sent: Friday, June 19, 2015 10:53 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] IHC turnaround time

Hi Histonetters,                                                                                                                                  6-19-15

We are wondering what the average turnaround times for IHC are for other labs.  We use the Biocare Intellipath instrumentation. It holds up to 50 slides ( 10 slides per 5 racks) with continuous feed per open rack.  All IHC orders placed by 12pm are usually out the same day by 5pm. We average 434 IHC slides per month and have a staff of 3 FTE's.

A pathologist has voiced concerns over our IHC output.  We are trying to determine "best practices" for IHC turnaround time as measured from time order placed to time of slide delivery.

If you could respond with your IHC TAT including number of techs and average of IHC slides monthly, we would appreciate any input you may be able to provide.

Thank you in advance,

Dawn Olszewski, HTL(ASCP)QIHC

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