[Histonet] IHC on fish
thoward at unm.edu
Mon Jun 15 11:59:47 CDT 2015
Why not do a Blast search for the sequences your anti-mammal antibodies recognize & see if they occur in fish? Some proteins are strongly conserved, or have conserved domains - you might get lucky! I tried a new (to me) antibody last week that was made against an amoeba, and it worked gangbusters on mouse and rat.
Dept. of Cell Biology & Physiology
University of New Mexico
Date: Sun, 14 Jun 2015 20:09:40 +1000
From: "Hardy, Cate" <chardy at csu.edu.au>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] IHC on fish
<144C8E2433CA3B4D9E080E5CDA803D05A752CC26FC at MAIL01.CSUMain.csu.edu.au>
Content-Type: text/plain; charset="us-ascii"
Has anyone performed IHC on fish. I have been asked to perform this using several of our routine antibodies that I use on mammals. My gut tells me it won't work but I am a lowly histotech and don't know anything. Seeking knowledge from learned tech's with experience in such matters
Senior Technical Officer
Veterinary Diagnostic Laboratory
Charles Strut University
Charles Sturt University
| ALBURY-WODONGA | BATHURST | CANBERRA | DUBBO | GOULBURN | MELBOURNE | ONTARIO | ORANGE | PORT MACQUARIE | SYDNEY | WAGGA WAGGA |
This email (and any attachment) is confidential and is intended for the use of the addressee(s) only. If you are not the intended recipient of this email, you must not copy, distribute, take any action in reliance on it or disclose it to anyone. Any confidentiality is not waived or lost by reason of mistaken delivery. Email should be checked for viruses and defects before opening. Charles Sturt University (CSU) does not accept liability for viruses or any consequence which arise as a result of this email transmission. Email communications with CSU may be subject to automated email filtering, which could result in the delay or deletion of a legitimate email before it is read at CSU. The views expressed in this email are not necessarily those of CSU.
Charles Sturt University in Australia http://www.csu.edu.au The Grange Chancellery, Panorama Avenue, Bathurst NSW Australia 2795 (ABN: 83 878 708 551; CRICOS Provider Numbers: 00005F (NSW), 01947G (VIC), 02960B (ACT)). TEQSA Provider Number: PV12018
Charles Sturt University in Ontario http://www.charlessturt.ca 860 Harrington Court, Burlington Ontario Canada L7N 3N4 Registration: www.peqab.ca
Consider the environment before printing this email.
More information about the Histonet