[Histonet] IHC on fish

[Tamara Howard]

Cate - 

Why not do a Blast search for the sequences your anti-mammal antibodies recognize & see if they occur in fish? Some proteins are strongly conserved, or have conserved domains - you might get lucky! I tried a new (to me) antibody last week that was made against an amoeba, and it worked gangbusters on mouse and rat.

Tamara

...................................................
Tamara Howard
Dept. of Cell Biology & Physiology
University of New Mexico
Albuquerque, NM





Message: 1
Date: Sun, 14 Jun 2015 20:09:40 +1000
From: "Hardy, Cate" <chardy at csu.edu.au>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] IHC on fish
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        <144C8E2433CA3B4D9E080E5CDA803D05A752CC26FC at MAIL01.CSUMain.csu.edu.au>
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Hi all;

Has anyone performed IHC on fish. I have been asked to perform this using several of our routine antibodies that I use on mammals. My gut tells me it won't work but I am a lowly histotech and don't know anything. Seeking knowledge from learned tech's with experience in such matters

Thanks

Cate Hardy
Senior Technical Officer
Veterinary Diagnostic Laboratory
Charles Strut University
NSW Australia
Charles Sturt University

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