[Histonet] IHC on fish

[Katy Milne]

I stained some zebra fish many many years ago.  For the life of me, I can't remember with what!

It's all going to come down to whether or not your Abs can recognize it.  I'd say give it a go and don't trust a negative result or look for Abs that should specifically work for fish.  Not all companies even test that obviously but there are a few that do.  I used to work for a company that would test Abs for western blots against pretty much anything that would go in a blender!  :)

What antigens are you looking for?

Good luck.

Katy

Message: 1
Date: Sun, 14 Jun 2015 20:09:40 +1000
From: "Hardy, Cate" <chardy at csu.edu.au>
To: "histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] IHC on fish
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Hi all;

Has anyone performed IHC on fish. I have been asked to perform this using several of our routine antibodies that I use on mammals. My gut tells me it won't work but I am a lowly histotech and don't know anything. Seeking knowledge from learned tech's with experience in such matters

Thanks

Cate Hardy
Senior Technical Officer
Veterinary Diagnostic Laboratory
Charles Strut University
NSW Australia
Charles Sturt University

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