[Histonet] IHC on fish
kmilne at bccrc.ca
Mon Jun 15 10:23:22 CDT 2015
I stained some zebra fish many many years ago. For the life of me, I can't remember with what!
It's all going to come down to whether or not your Abs can recognize it. I'd say give it a go and don't trust a negative result or look for Abs that should specifically work for fish. Not all companies even test that obviously but there are a few that do. I used to work for a company that would test Abs for western blots against pretty much anything that would go in a blender! :)
What antigens are you looking for?
Date: Sun, 14 Jun 2015 20:09:40 +1000
From: "Hardy, Cate" <chardy at csu.edu.au>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] IHC on fish
<144C8E2433CA3B4D9E080E5CDA803D05A752CC26FC at MAIL01.CSUMain.csu.edu.au>
Content-Type: text/plain; charset="us-ascii"
Has anyone performed IHC on fish. I have been asked to perform this using several of our routine antibodies that I use on mammals. My gut tells me it won't work but I am a lowly histotech and don't know anything. Seeking knowledge from learned tech's with experience in such matters
Senior Technical Officer
Veterinary Diagnostic Laboratory
Charles Strut University
Charles Sturt University
| ALBURY-WODONGA | BATHURST | CANBERRA | DUBBO | GOULBURN | MELBOURNE |
| ONTARIO | ORANGE | PORT MACQUARIE | SYDNEY | WAGGA WAGGA |
More information about the Histonet