[Histonet] Patti Loykasek - k/L staining

[Cindy Bulmer]

Hi Tim,I use the Dako Kappa and Lambda, Poly Abs.  With the Kappa, my titer is 1:80,000 and the Lambda is 1:250,000.  I use the Innovex Rc Receptor Blocker as the diluent, this seems to cut down on a lot of the background.  My Pathologists are very happy with thesestains.  I use tonsil for my positive controls.Hope this helps,
Cindy Bulmer HT(ASCP), QIHCIHC Supervisor, CTPLWaco, TX
 


     On Monday, June 1, 2015 3:43 PM, "Morken, Timothy" <Timothy.Morken at ucsf.edu> wrote:
   

 Patti, do remember any details of the following procedure" We have a heart pathologist  interested....

Thanks to you or anyone else that can help with this.


++++++++++++++
>From 2005...

Kappa and lambda are certainly difficult to interpret. They both can be
present circulating in the serum, thus the "background" staining in most
tissue sections. We use polyclonal antibodies, and get quite good results.
We use a tonsil as a control as bone marrows are just too precious & small
for us to use. We look for the plasma cells to be staining with a dark
cytoplasmic stain & a slight "blush" to the germinal centers of the tonsil.
It does take a pathologist skilled in interpreting them. A true positive is
in the cytoplasm of the cells, not in-between the cells (due to circulating
K/L). We do use one titer for lymph nodes, and another for bone marrows & GI
biopsies.  Plus a different titer & pretreatment if we're looking for
amyloid.
Well, I hope I've helped & not confused you. I'd be happy to do my best to
answer any questions that you have.

Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA
+++++++++++++++++++++++++++++++++++++++++++++++++++

Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
505 Parnassus Ave, Box 1656
Room S570
San Francisco, CA 94143

(415) 353-1266 (ph)
(415) 514-3403 (fax)
tim.morken at ucsfmedctr.org<mailto:tim.morken at ucsfmedctr.org>

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