[Histonet] Histonet Digest, Vol 140, Issue 9

[Leslie, Mary]

Taking a poll:  How many of your labs recycle formalin, alcohol and xylene?  

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Subject: Histonet Digest, Vol 140, Issue 9

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Today's Topics:

   1. 2 Open Positions in Little Rock Arkansas (Marcum, Pamela A)
   2. Gross Room QC sheet (Histology Technician)
   3. Medica ELGA DV 25 (Histology Technician)
   4. Roche B-gal Staining Kit (Coffey, Anna (NIH/NCI) [C])


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Message: 1
Date: Thu, 9 Jul 2015 15:15:47 +0000
From: "Marcum, Pamela A" <PAMarcum at uams.edu>
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] 2 Open Positions in Little Rock Arkansas
Message-ID: <dc5416a8415544ceb9cca4c9f981cec5 at MAIL13M2N2.ad.uams.edu>
Content-Type: text/plain; charset="us-ascii"

WE currently have two open positions for either registered HT or HTL.  Registry must be current and some experience.  We are a heavily computerized Histology Laboratory and will require the persons applying to be comfortable with computers.  We are a routine Histology Laboratory at the University of Arkansas for Medical Sciences, in Little Rock.  This is a beautiful area in Central Arkansas with lots of things to do both indoors and especially outdoors.   If you are interested please contact me with your resume as soon as possible.


Please no recruiters, we are not allowed to use you,  I am sorry.

Thank You,

Pam Marcum

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Message: 2
Date: Thu, 9 Jul 2015 16:20:09 +0000 (UTC)
From: Histology Technician <histology81176 at att.net>
To: Histonet <histonet at lists.utsouthwestern.edu>,
	"histonet-request at lists.utsouthwestern.edu"
	<histonet-request at lists.utsouthwestern.edu>
Subject: [Histonet] Gross Room QC sheet
Message-ID:
	<1290933179.1997863.1436458809680.JavaMail.yahoo at mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

 Does anyone have a Gross Room QC sheet you'd like to share?? Thanks so much!

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Message: 3
Date: Thu, 9 Jul 2015 16:22:47 +0000 (UTC)
From: Histology Technician <histology81176 at att.net>
To: Histonet <histonet at lists.utsouthwestern.edu>,
	"histonet-request at lists.utsouthwestern.edu"
	<histonet-request at lists.utsouthwestern.edu>
Subject: [Histonet] Medica ELGA DV 25
Message-ID:
	<377965148.2007366.1436458967925.JavaMail.yahoo at mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

 Does anyone use the DI water system called Medica by Eqova?? We just recently got 2 in our lab and was wondering if anyone had an SOP you'd like to share?? Can you tell my what daily QC needs to be done with it?? Water tested?? Thanks!

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Message: 4
Date: Thu, 9 Jul 2015 16:57:14 +0000
From: "Coffey, Anna (NIH/NCI) [C]" <anna.coffey at nih.gov>
To: "histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Roche B-gal Staining Kit
Message-ID: <5C3E10119A1B824FBE92B08279F74A910259809B at msgb10.nih.gov>
Content-Type: text/plain; charset="us-ascii"

Hello,

I have been asked to do a B-gal enzyme stain using a Roche kit on frozen mouse sections. Another histologist recommended post-fixing in a glutaraldehyde-formaldehyde solution although this isn't mentioned in the kit instructions. This has me worried that there may be other things missing from the kit instructions that could improve the results. Has anyone else had any experience using this kit and, if so, did you find that other alterations to the protocol were required? Also wondering about potential counterstains that might work with this one. I'd appreciate any pointers if you have them!

Thanks!
Anna

Anna Coffey, MS, HTL(ASCP)CM
Histotechnologist
Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc.
Bld 539, 224
Frederick, Maryland 21702
anna.coffey at nih.gov
301-846-1730



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End of Histonet Digest, Vol 140, Issue 9
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