[Histonet] Re. Decalcification with formic acid sodium

Hobbs, Carl carl.hobbs at kcl.ac.uk
Sun Jul 26 13:38:33 CDT 2015

Mouse knee joints:
done lots of decalcified FFPWS for assessment of articular cartilage degeneration models.
See Histonet images for a Tol blue image.
Decal in 10 % EDTA for 3 days on a rocker at RT.
Sure....5days if you are worried.
No difference in Immuno-reactivity, imho.
If you want to use buffered Formic acid, use Formic acid; sodium formate.
Use of citric acid with Formic acid does not make a buffer.
It's just mixing two relatively mild acids.....
However, I am sure that Prof Kiernan can further enlighten us.

Carl Hobbs FIBMS 
Histology and Imaging Manager 
Wolfson CARD 
Guys Campus, London Bridge  
Kings College London 
SE1 1UL 
020 7848 6813    

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