[Histonet] Eosin

[Monson, Frederick]

Long ago, in the foggy, foggy past,  we were admonished to insure that our Eosin in the bottle had a slight 'green' tinge in good daylight.

When we finally got fluorescence, we would check an H&E-stained section with the fluorescein filters to insure the fluorescence of the Eosin. 

Very scientific, I know, but when the 'green' was gone, the Eosin was replaced.

Cheers,

Fred Monson

Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of Pennsylvania
Schmucker Science South - Room SSS-024
MailDrop:  Geology-Astronomy
750 South Church Street
West Chester, PA, 19383
610-738-0437
fmonson at wcupa.edu

-----Original Message-----
From: Kienitz, Kari [mailto:kkienitz at orclinic.com] 
Sent: Monday, July 13, 2015 11:21 AM
To: Hannen, Valerie; Histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Eosin

It's always kind of a stab in the dark to diagnose H&E problems without a little more information.  One thing I would try if you don't feel its an actual Eosin problem is your alcohol prior to the eosin.  Slide volume and humidity can dilute your alcohol and cause lighter cytoplasmic staining.  The alcohol prior to eosin should be 95% and changed daily if need be.


Kari Kienitz HT, (ASCP)
Histology Laboratory
Gastroenterology-EAST
The Oregon Clinic
1111 NE 99th Ave
Portland, OR  97220
503.935.8311
kkienitz at orclinic.com




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From: Hannen, Valerie [Valerie.Hannen at parrishmed.com]
Sent: Monday, July 13, 2015 8:05 AM
To: Histonet at lists.utsouthwestern.edu
Subject: [Histonet] Eosin

Good morning,

I once again am dealing with a my picky Pathologist!!  About a year ago, he started to complain about not having enough Eosin on his sections( first morning rack on Monday).. I went from changing the Eosin on Friday and stirring it on Monday to totally changing it  on Monday...it has all been good until the end of last week. The problem has started up again... little Eosin in the first rack of Monday morning.  Any suggestions??

Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.hannen at parrishmed.com<mailto:valerie.hannen at parrishmed.com>
www.parrishmed.com

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