[Histonet] Eosin

[Hannen, Valerie]

Rene,

As I stated before and maybe I was not entirely clear, we had this problem ( little Eosin in the sections of the first rack of slides on Monday morning) before, but had remedied it by starting to change the Eosin on Monday morning before the first rack of slides was run.  He now is stating that the problem of little Eosin in the sections from the first rack on Monday A.M. is starting again.  Since the last fix of the problem we have not deviated in any way, procedure or chemical wise.  We have used the same lot # in previous weeks for the Eosin as we currently have on the stainer ( I changed the Eosin this morning) , so I don’t think it is an Eosin problem per se.

Kari,

We do use 95% alcohol before our Eosin at all times.  I would think that if the alcohol before the  Eosin was the problem, all of my racks would have the “little Eosin in the sections” problem.. not just the first rack.

Again any and all suggestions are welcomed!!

Thanks again Rene and Kari for replying.

Valerie

From: Rene J Buesa [mailto:rjbuesa at yahoo.com]
Sent: Monday, July 13, 2015 11:20 AM
To: Hannen, Valerie; Histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Eosin

It is difficult to advise you anything without knowing what is your PT's complaint. What he does complain about?
René


On Monday, July 13, 2015 11:17 AM, "Hannen, Valerie" <Valerie.Hannen at parrishmed.com<mailto:Valerie.Hannen at parrishmed.com>> wrote:

Good morning,

I once again am dealing with a my picky Pathologist!!  About a year ago, he started to complain about not having enough Eosin on his sections( first morning rack on Monday).. I went from changing the Eosin on Friday and stirring it on Monday to totally changing it  on Monday...it has all been good until the end of last week. The problem has started up again... little Eosin in the first rack of Monday morning.  Any suggestions??

Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.hannen at parrishmed.com<mailto:valerie.hannen at parrishmed.com><mailto:valerie.hannen at parrishmed.com<mailto:valerie.hannen at parrishmed.com>>
www.parrishmed.com<http://www.parrishmed.com>

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