[Histonet] Decalcification of mouse jaw and legs

Swartwood, Steven J Steven.Swartwood at cshs.org
Fri Jul 10 16:52:47 CDT 2015

Hello all,

I hope everyone is having or had a fantastic week,

I'm going to test a few different decal methods on mouse jaws and leg bones. I was wondering if anyone does this routinely out there who is willing to share a protocol. I've never done this on mouse jaw/leg bones before. I've only done this on human tissues. From what I've read it seems that for routine histology low % HCl is fine, but if I wanted to perform IHC then anywhere from 5-15% formic acid may be the best way to go. Nitric acid is probably too strong of a decal agent for these tiny specimens I would assume. Maybe an EDTA based decal agent may be best as well. I'm just really inexperienced with this and I'm very open to ideas and trying a few different methods.

Steven Swartwood HT(ASCP)
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