[Histonet] Coverslipping mystery

[Jennifer MacDonald]

What type of clearing agent are you using?  The aliphatic hydrocarbons are 
not compatible with all mounting media.



From:   Adam Boanas <a.boanas at epistem.co.uk>
To:     "histonet at lists.utsouthwestern.edu" 
<histonet at lists.utsouthwestern.edu>
Date:   07/09/2015 06:16 AM
Subject:        [Histonet] Coverslipping mystery



Hello,

We are having a problem that is developing into a big issue in our lab and 
I was wondering if anybody could shed any light on it. Our CV5000 
coverslipper has recently started introducing microscopic air bubbles onto 
the slides during coverslipping. We have been told by our engineer that it 
is a consequence of the age and use of the motor and that sourcing another 
for an instrument that old (15yrs) will be v difficult. As such, we have 
been forced to manually coverslip using DPX and a pipette - manually 
applying the coverslips to the slide, thus mirroring the action of the 
coverslipper. This is fine at first and for the next few days the slides 
look great and very clean. However, after about day 4 -5 days post 
coverslipping, the slides develop an odd appearance down the microscope 
which looks like very fine `parched earth / crazy paving` all over the 
slide - including the section. The excess mountant around the edge of the 
coverslip also has a very faint, cloudy appearance whe
 n this occurs. This of course renders the slide un-useable. Does anyone 
have a clue what this might be down to / how we can stop it?
We are struggling for ideas with this one!  - this occurs with fresh DPX 
also.

Many thanks
Adam

Adam Boanas
Senior Research Associate
Epistem Ltd
48 Grafton Street
Manchester, M13 9XX

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