[Histonet] Coverslipping mystery

Simmons, Christopher simmca at UPMC.EDU
Thu Jul 9 08:49:00 CDT 2015

Look in your manual, near the back. There should be mountant type calibration settings for yo to adjust the machine to.
That, hopefully will solve your issue!

Chris Simmons B.S., A.S., HTL(ASCP)
Supervisor, UPP Dermatopathology
412.864.3880 office
412.612.0881 cell

-----Original Message-----
From: Rene J Buesa [mailto:rjbuesa at yahoo.com] 
Sent: Thursday, July 09, 2015 9:47 AM
To: Adam Boanas; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Coverslipping mystery

Just guessing, but I think the "mystery" is caused by how fluid the DPX is. Guessing again, but it probably is more dense as it should.I would dilute it to the lowest density it can be used in your coverslipper. Give it a try.René 

     On Thursday, July 9, 2015 9:40 AM, Adam Boanas <a.boanas at epistem.co.uk> wrote:


We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance when this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it?
We are struggling for ideas with this one!  - this occurs with fresh DPX also.

Many thanks

Adam Boanas
Senior Research Associate
Epistem Ltd
48 Grafton Street
Manchester, M13 9XX

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