[Histonet] Tissue Processor Validation Procedure

[Johnson, Carole]

Hello Allison,

When we purchased a new processor, I chose tissue specimens representative of what we normally see in our lab (surgical/biopsy and necropsy tissues) from cases which had been signed out within the previous 48-72 hours. I then ran parallel samples on both processors (same protocols, run the same day, fresh reagents in both processors) and then set up a grading scale for the pathologists to do a blind comparison. After that, I did a (brief) statistical analysis to determine if there was any significant difference between slides from the two processors. For IHC (we are a small lab with a short IHC menu currently), I ran parallel Vimentin and Multicytokeratin on the same blocks as used for the H&E and did a comparison just as for the H&E slides. I presented the final data to my chief pathologist who then signed off on the validation. I keep a copy of the validation with my equipment records. I hope this helps.

Best regards,
Carole

Carole Johnson
Carole Johnson, HT(ASCP)cm
New Mexico Department of Agriculture
Veterinary Diagnostic Services
505.383.9299

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