[Histonet] RE: Amyloid by Congo Red

[Preiszner, Johanna]

Hi,


I can send amyloid positive control tissue block (cerebral amyloid vasculitis), just send me an email to 
preiszne <@t> etsu.edu with your mailing address. Very nice staining, tissue was harvested two month ago.
We have more than we'll ever use up.

Cheers,
Hanna


________________________________________
From: Jeffrey Robinson [JRobinson <@t> pathology-associates.com]
Sent: Friday, January 23, 2015 3:33 PM
To: Preiszner, Johanna
Subject: RE: Amyloid by Congo Red

HI Hanna-  you are the second tech suggesting the Sigma kit so I may try that.  I have an old Leitz fluorescent scope that is still around somewhere that I used when we did kidney bx's but we are in a new building now without a fluorescent room so I don't think I can set it up.  Thanks!  Jeff.

-----Original Message-----
From: Preiszner, Johanna [mailto:PREISZNE <@t> mail.etsu.edu]
Sent: Friday, January 23, 2015 12:27 PM
To: Jeffrey Robinson
Subject: RE: Amyloid by Congo Red

Hi,

we use the Sigma Kit, and never a had problem. Protocol is different than what you describe so you may want to try it.

Also what Sarah mentioned: might need to check the stain on a fluorescence microscope with excitation at 497nm. The emitted signal is very red and we never had non-specific staining. I can send pictures if interested.

Hanna Preiszner
ETSU/QCOM


________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Jeffrey Robinson [JRobinson <@t> pathology-associates.com]
Sent: Friday, January 23, 2015 12:43 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Amyloid by Congo Red

Greetings to all Histotechs-  Here's an amyloid question for the braintrust.  We are cutting our slides and controls at 9 and staining in Congo Red for 1 hour.  The control stains fine but the patient tissue is staining negative even on cases that the pathologist assures us should be positive for amyloid.  We are using the Leica APEX charged slides with control and patient tissue on the same slide.  Does anyone have any thoughts on why the patient tissue is not staining?  Thanks!

Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.


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