[Histonet] Paraffin-Plastic Stratification/Congo red problem

[Michael Ann Jones]

I also noticed stain fading with time - controls cut way ahead of staining?
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones <@t> metropath.com




On 1/23/15, 1:00 PM, "Wheelock, Timothy R." <twheelock <@t> mclean.harvard.edu>
wrote:

>Hi Jeffery:
>
>Yes, I notice the same "paraffin dust bunnies", perhaps especially since
>I use a embedding paraffin that has a fair amount of plastic.
>Before I embed my brain tissue, I mix the embedding media thoroughly,
>until the solution is clear.
>
>As for your Congo Red problem, I wish that I could help you.
>I am now experiencing the same problem, except that it affects the
>positive controls as well.
>The staining is there, but much fainter than it should be.
>
>I usually immerse albumin coated slides in 1% Congo red for 15 minutes,
>differentiate them with Potassium Hydroxide for 3 dips, counterstain with
>Harris Hematoxylin for 1 minute, differentiate with acid alcohol for 5-10
>dips, immerse in running tap water for 10 minutes, immerse in 95% ethanol
>for 2 dips, then dehydrate and mount.
>
>Tim
>
>
>
>
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