[Histonet] HTL exam
Joelle Weaver
joelleweaver <@t> hotmail.com
Fri Jan 23 14:13:25 CST 2015
Agree with your points and book recommendations. I do remember some slide ID on mine. Also there are more taxonomy II & III and operations on the HTL, which warrants a slightly different study approach, but completely acheivable.
Joelle Weaver MAOM, HTL (ASCP) QIHC
> From: JRobinson <@t> pathology-associates.com
> To: scbymar <@t> yahoo.com; histonet <@t> lists.utsouthwestern.edu
> Date: Fri, 23 Jan 2015 19:08:47 +0000
> Subject: RE: [Histonet] HTL exam
> CC:
>
> I used the NSH booklets on the various Histology subjects. I don't know about their current availability- I think they were on a CD now but I haven't checked lately. I learned a lot from the booklets as they not only give the correct answer they also described why the other answers were wrong along with some background pertaining to related subjects. With the test being online now I don't know how important the color plates of the various special stains are but I found it extremely helpful to know all of the stains by sight backwards and forwards- even stains that we did not run in our lab as there were a lot of questions that would refer to different methods of staining for the same structure or organism, etc. I used Sheehan and Bancroft as my texts. Bancroft is British so there is a different slant to his writing that I find interesting. I have read Carson's but I do not feel it has enough background information. Lee Luna's last book has great color plates but the organization is poor and it can be hard to find things- I think someone finished it up after he passed away.
>
> Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Maryann Morissette
> Sent: Friday, January 23, 2015 10:43 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] HTL exam
>
>
> Hi all. Was just wondering if anyone has just taken the HTL exam. I passed the HT with just reading an older Frieda Carson book. Can someone give me some advice on books that really helped them? Thanks!
> Sent from my iPhone
>
> > On Jan 23, 2015, at 1:01 PM, histonet-request <@t> lists.utsouthwestern.edu wrote:
> >
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> > Today's Topics:
> >
> > 1. RE: rodent eye (Gowan,Christie C)
> > 2. Cracking paraffin blocks (Wheelock, Timothy R.)
> > 3. RE: Cheap Disposable Blades for Facing In (Bea DeBrosse-Serra)
> > 4. Re: Cracking paraffin blocks (Hans B Snyder)
> > 5. Amyloid by Congo Red (Jeffrey Robinson)
> > 6. Thermo IHC (Cheri Miller)
> > 7. Problem with cracked paraffin blocks (Wheelock, Timothy R.)
> > 8. Re: Amyloid by Congo Red (Michael Ann Jones)
> >
> >
> > ----------------------------------------------------------------------
> >
> > Message: 1
> > Date: Wed, 21 Jan 2015 16:23:06 +0000
> > From: "Gowan,Christie C" <christiecgowan <@t> dermatology.med.ufl.edu>
> > Subject: RE: [Histonet] rodent eye
> > To: Casie Phillips <casie4384 <@t> gmail.com>,
> > "histonet <@t> lists.utsouthwestern.edu"
> > <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> > <CCC0568455733548A03568AC55691F762596DA <@t> AHC-MB02.ad.ufl.edu>
> > Content-Type: text/plain; charset="us-ascii"
> >
> > Hi Casie,
> > Hope by now you have rec'd some good tips on rodent eye prep. The only thing I have to offer is that we always used Davidson's fixative for 24 hours and then transferred to 70% ETOH. This worked beautifully preserving all eye components. Good luck and don't forget to check the Histonet archives where I know rodent eyes have been discussed in the past.
> >
> > Christie Gowan HT (ASCP)
> >
> > Department of Dermatology
> > 4037 NW 86th Terrace, 4th Fl
> > Mohs Laboratory
> > Gainesville, FL 32606
> > Phone: 352 594-1529
> >
> >
> > ________________________________________
> > From: histonet-bounces <@t> lists.utsouthwestern.edu
> > [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Casie
> > Phillips [casie4384 <@t> gmail.com]
> > Sent: Thursday, January 15, 2015 2:53 PM
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: [Histonet] rodent eye
> >
> > Good afternoon,
> >
> > I am currently working with Lewis rats performing corneal alkali
> > injuries at varying strengths. Is there someone there that has prior
> > experience working with a rat eye and would be willing to share
> > information on the most effective ways to preserve, fix and cut the cornea sample.
> >
> > We are interested in using the cornea without using the whole globe if
> > possible. For now we will be using basic H&E staining with a
> > possibility of immunohistochemistry at a later time. The main outcome
> > we are looking for is to find the presence of neutrophils in the
> > cornea. A second objective is to look for any damaged or newly reconstructed tissue.
> >
> > I would greatly appreciate any advice relating to the type of paraffin
> > used, the ideal length of time to save the tissue and any assistance
> > you can suggest for completing this process successfully.
> >
> > Thank you for your time. Any assistance will be greatly appreciated.
> >
> > Sincerely,
> >
> > Casie Phillips
> > Casie4384 <@t> gmail.com
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> > ------------------------------
> >
> > Message: 2
> > Date: Fri, 23 Jan 2015 14:57:15 +0000
> > From: "Wheelock, Timothy R." <twheelock <@t> mclean.harvard.edu>
> > Subject: [Histonet] Cracking paraffin blocks
> > To: "'Histonet <@t> lists.utsouthwestern.edu'"
> > <Histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> > <69718C0B0B3C414D9F8E7214AD400CC9773EABC4 <@t> PHSX10MB11.partners.org>
> > Content-Type: text/plain; charset="us-ascii"
> >
> > Hi Everyone:
> >
> > Recently, I purchased the Medite Valida Embedding Center, which I demoed previously without a problem, if I recall right.
> > I am having problems now with blocks developing cracks on the cold plate.
> > The cracks run either through the wax right next to the specimen, or more frequently, right through the tissue itself.
> > I use Surgipath Embedding Media (EM-400).
> > The surface of the Valida's cold plate is -14C.
> >
> > The company has not seen this happening before, but they are looking into this further.
> > Also, I had the same problem when I demoed the Thermo-Fisher HistoStar.
> > I do not think that this is a problem inherent with any particular machine.
> > Has anyone encountered this problem before?
> > If so, how did you resolve it?
> >
> > Is it possible that the -14C cold plate is too cold? Should I warm it up a bit?
> > The surface of the Valida cold plate is, I believe, made out of smooth stainless steel.
> > My old (24 years) Shandon Embedding Center's cold plate is made out of cast aluminum and has a slightly "rougher" texture.
> > Could this produce a different way of conducting heat out of the paraffin block than the Shandon?
> > Perhaps the stainless steel draws heat from the blocks faster than the aluminum, and thus causes the cracking?
> >
> > I have used the Shandon Embedding Center for over 24 years.
> > I have never had a problem with blocks that crack.
> >
> > Thanks for any thoughts that you may have on this.
> >
> > Tim Wheelock
> > Harvard Brain Bank
> > McLean Hospital
> > Belmont, MA
> >
> >
> > The information in this e-mail is intended only for the person to whom
> > it is addressed. If you believe this e-mail was sent to you in error
> > and the e-mail contains patient information, please contact the
> > Partners Compliance HelpLine at http://www.partners.org/complianceline
> > . If the e-mail was sent to you in error but does not contain patient
> > information, please contact the sender and properly dispose of the e-mail.
> >
> >
> > ------------------------------
> >
> > Message: 3
> > Date: Fri, 23 Jan 2015 15:49:27 +0000
> > From: Bea DeBrosse-Serra <BDeBrosse-Serra <@t> isisph.com>
> > Subject: RE: [Histonet] Cheap Disposable Blades for Facing In
> > To: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>, Sandra Cheasty
> > <cheastys <@t> svm.vetmed.wisc.edu>
> > Cc: "Histonet \(histonet <@t> lists.utsouthwestern.edu\)"
> > <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> > <CB69CD3FBEB5524CA851930C971288FD329F1252 <@t> EXCH10MB01.isis.local>
> > Content-Type: text/plain; charset="us-ascii"
> >
> > We are doing the same.
> >
> > Beatrice DeBrosse-Serra HT(ASCP)QIHC
> > Isis Pharmaceuticals
> > Antisense Drug Discovery
> > 2855 Gazelle Ct.
> > Carlsbad, CA 92010
> > 760-603-2371
> >
> >
> >
> > -----Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu
> > [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> > Jennifer MacDonald
> > Sent: Friday, January 23, 2015 2:59 AM
> > To: Sandra Cheasty
> > Cc: Histonet (histonet <@t> lists.utsouthwestern.edu)
> > Subject: Re: [Histonet] Cheap Disposable Blades for Facing In
> >
> >
> > We save our blade from the previous day to use for facing. We keep them in slide mailers.
> >
> > Sent from my iPad
> >
> >>> On Jan 22, 2015, at 4:21 PM, Sandra Cheasty
> >> <cheastys <@t> svm.vetmed.wisc.edu> wrote:
> >>
> >> Hello everyone,
> >>
> >> Does anyone have a source for cheap, low-profile
> >> blades
> > for facing in blocks?
> >>
> >> Thanks!
> >> Sandy
> >>
> >>
> >>
> >> Sandra J. Cheasty, HT (ASCP)
> >>
> >> Histology & Necropsy Supervisor, President Keith Richards Fan Club
> >>
> >> UW-Madison, School of Veterinary Medicine
> >>
> >>
> >>
> >>
> >>
> >>
> >>
> >>
> >>
> >> _______________________________________________
> >> Histonet mailing list
> >> Histonet <@t> lists.utsouthwestern.edu
> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> > ------------------------------
> >
> > Message: 4
> > Date: Fri, 23 Jan 2015 12:07:03 -0500
> > From: Hans B Snyder <hans <@t> histologistics.com>
> > Subject: Re: [Histonet] Cracking paraffin blocks
> > To: "Wheelock, Timothy R." <twheelock <@t> mclean.harvard.edu>
> > Cc: "Histonet <@t> lists.utsouthwestern.edu"
> > <Histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> >
> > <CAAYBjcuB0-v=SwDCRjt5bpu=yegTuDoWm3_2AtdSCt4iJTp_tw <@t> mail.gmail.com>
> > Content-Type: text/plain; charset=UTF-8
> >
> > Hello Tim,
> >
> > I also have had this cracking problem in the past but since resolved.
> > I'm not sure about the specifics of the cracking problem but the cold
> > plate might have something to do with it. Our cold plate is set to
> > -5C and use Paraplast from McCormick Scientific. Our paraffin is
> > roughly the same melting temp as yours, so probably not much
> > difference.
> >
> > Unfortunately trial and error is apart of our jobs since there is
> > relatively little uniformity in histology equipment and products. Have
> > you tried setting the cold plate to a warmer temperature yet? It's
> > worth a try and if that doesn't work maybe the paraffin has been
> > getting too hot. I know when the paraffin gets too hot, the wax and
> > plastic separate or break down and cause inconsistencies in the
> > paraffin. To test this, take an external thermometer and place inside
> > the paraffin tank. Then record the temp every hour for some time.
> > This will tell you if the tank itself is a consistent temperature.
> >
> > Also, are the blocks taken off the cold plate and immediately jarred
> > loose from the mold? Sometimes when I do this the shock of pried out
> > of the mold can cause the paraffin to become brittle and break rather
> > than bend.
> >
> > Let me know how it goes.
> >
> > Thank you
> > Hans B Snyder
> > Histologistics
> > 60 Prescott Street
> > Worcester, MA 01605
> > 508-308-7800
> > hans <@t> histologistics.com
> >
> >
> > On Fri, Jan 23, 2015 at 9:57 AM, Wheelock, Timothy R.
> > <twheelock <@t> mclean.harvard.edu> wrote:
> >> Hi Everyone:
> >>
> >> Recently, I purchased the Medite Valida Embedding Center, which I demoed previously without a problem, if I recall right.
> >> I am having problems now with blocks developing cracks on the cold plate.
> >> The cracks run either through the wax right next to the specimen, or more frequently, right through the tissue itself.
> >> I use Surgipath Embedding Media (EM-400).
> >> The surface of the Valida's cold plate is -14C.
> >>
> >> The company has not seen this happening before, but they are looking into this further.
> >> Also, I had the same problem when I demoed the Thermo-Fisher HistoStar.
> >> I do not think that this is a problem inherent with any particular machine.
> >> Has anyone encountered this problem before?
> >> If so, how did you resolve it?
> >>
> >> Is it possible that the -14C cold plate is too cold? Should I warm it up a bit?
> >> The surface of the Valida cold plate is, I believe, made out of smooth stainless steel.
> >> My old (24 years) Shandon Embedding Center's cold plate is made out of cast aluminum and has a slightly "rougher" texture.
> >> Could this produce a different way of conducting heat out of the paraffin block than the Shandon?
> >> Perhaps the stainless steel draws heat from the blocks faster than the aluminum, and thus causes the cracking?
> >>
> >> I have used the Shandon Embedding Center for over 24 years.
> >> I have never had a problem with blocks that crack.
> >>
> >> Thanks for any thoughts that you may have on this.
> >>
> >> Tim Wheelock
> >> Harvard Brain Bank
> >> McLean Hospital
> >> Belmont, MA
> >>
> >>
> >> The information in this e-mail is intended only for the person to
> >> whom it is addressed. If you believe this e-mail was sent to you in
> >> error and the e-mail contains patient information, please contact the
> >> Partners Compliance HelpLine at
> >> http://www.partners.org/complianceline . If the e-mail was sent to
> >> you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail.
> >> _______________________________________________
> >> Histonet mailing list
> >> Histonet <@t> lists.utsouthwestern.edu
> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> > ------------------------------
> >
> > Message: 5
> > Date: Fri, 23 Jan 2015 17:43:33 +0000
> > From: Jeffrey Robinson <JRobinson <@t> pathology-associates.com>
> > Subject: [Histonet] Amyloid by Congo Red
> > To: "histonet <@t> lists.utsouthwestern.edu"
> > <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> >
> > <204A03EB5A7F0A4BB1EEDD52A963829C16D8B360 <@t> PAEXCH1.PathologyAssociates.
> > local>
> >
> > Content-Type: text/plain; charset="us-ascii"
> >
> > Greetings to all Histotechs- Here's an amyloid question for the braintrust. We are cutting our slides and controls at 9 and staining in Congo Red for 1 hour. The control stains fine but the patient tissue is staining negative even on cases that the pathologist assures us should be positive for amyloid. We are using the Leica APEX charged slides with control and patient tissue on the same slide. Does anyone have any thoughts on why the patient tissue is not staining? Thanks!
> >
> > Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.
> >
> >
> > This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you.
> >
> >
> > ------------------------------
> >
> > Message: 6
> > Date: Fri, 23 Jan 2015 11:54:06 -0600
> > From: Cheri Miller <cmiller <@t> physlab.com>
> > Subject: [Histonet] Thermo IHC
> > To: "histonet <@t> lists.utsouthwestern.edu"
> > <histonet <@t> lists.utsouthwestern.edu>
> > Cc: "histonet-bounces <@t> lists.utsouthwestern.edu"
> > <histonet-bounces <@t> lists.utsouthwestern.edu>
> > Message-ID: <E3C81A010935EA41B379AC765103F3BF4000D6C102 <@t> olsrv12>
> > Content-Type: text/plain; charset="iso-8859-1"
> >
> > Hi Histonetters! I need some help. We just acquired a Thermo IHC
> > system. Can anyone help us cut through the *****88*888 and give me
> > some helpful tips? We have practical experience on the Ventana
> > systems only. Has anyone been successful at using only 1 HIER buffer?
> > Any tips on how to shorten the offline retrieval process? Currently we
> > are at over 40 mins. Seems to me a ph of 7.6 -7.8 should work for most
> > all of our antibodies. Thanks, Cheri
> >
> > Cheryl A. Miller HT ASCP cm
> > Physicians Laboratory, P.C.
> > 4840 F St.
> > Omaha , NE. 68117
> > 402 731 4145 ext. 532
> > Cell 402 980 2537
> > Fax 402 731 8653
> >
> > ________________________________
> > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system.
> >
> >
> > ------------------------------
> >
> > Message: 7
> > Date: Fri, 23 Jan 2015 17:55:12 +0000
> > From: "Wheelock, Timothy R." <twheelock <@t> mclean.harvard.edu>
> > Subject: [Histonet] Problem with cracked paraffin blocks
> > To: "'histonet <@t> lists.utsouthwestern.edu'"
> > <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> > <69718C0B0B3C414D9F8E7214AD400CC9773EAC56 <@t> PHSX10MB11.partners.org>
> > Content-Type: text/plain; charset="us-ascii"
> >
> > Hi again everyone:
> >
> > I want to thank you all for your advice.
> > The consensus is that I have my new embedding center's cold plate set way too low at -14C, and that I should try raising it to around -5C to cure my cracked block problem.
> > I will run some test blocks over the weekend, and then embed them at this new temperature on Monday.
> > Otherwise my Valida embedding center is working very nicely (as did the HistoStar when I demoed it).
> > The cassette holding tank can accommodate a large number of brain specimens and the controls are very easy to use.
> > Thanks again for your advice.
> > Have a great weekend.
> >
> > Tim
> >
> > Tim Wheelock
> > Harvard Brain Bank
> > McLean Hospital
> > Belmont, MA
> >
> >
> > The information in this e-mail is intended only for the person to whom
> > it is addressed. If you believe this e-mail was sent to you in error
> > and the e-mail contains patient information, please contact the
> > Partners Compliance HelpLine at http://www.partners.org/complianceline
> > . If the e-mail was sent to you in error but does not contain patient
> > information, please contact the sender and properly dispose of the e-mail.
> >
> >
> > ------------------------------
> >
> > Message: 8
> > Date: Fri, 23 Jan 2015 17:58:31 +0000
> > From: Michael Ann Jones <mjones <@t> metropath.com>
> > Subject: Re: [Histonet] Amyloid by Congo Red
> > To: Jeffrey Robinson <JRobinson <@t> pathology-associates.com>,
> > "histonet <@t> lists.utsouthwestern.edu"
> > <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID: <D0E7D992.65C3%mjones <@t> metropath.com>
> > Content-Type: text/plain; charset="us-ascii"
> >
> > What protocol and reagents are you using for the stain?
> > Michael Ann Jones, HT (ASCP)
> > Histology Manager
> > Metropath
> > 7444 W. Alaska Dr. #250
> > Lakewood, CO 80226
> > 303.634.2511
> > Mjones <@t> metropath.com
> >
> >
> >
> >
> >
> >
> > On 1/23/15, 10:43 AM, "Jeffrey Robinson"
> > <JRobinson <@t> pathology-associates.com> wrote:
> >
> >> Greetings to all Histotechs- Here's an amyloid question for the
> >> braintrust. We are cutting our slides and controls at 9 and staining
> >> in Congo Red for 1 hour. The control stains fine but the patient
> >> tissue is staining negative even on cases that the pathologist
> >> assures us should be positive for amyloid. We are using the Leica
> >> APEX charged slides with control and patient tissue on the same
> >> slide. Does anyone have any thoughts on why the patient tissue is not staining? Thanks!
> >>
> >> Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology
> >> Lab, Clovis, CA.
> >>
> >>
> >> This email and attachments may contain PHI that is privileged and
> >> confidential and is not intended for any unauthorized person. If you,
> >> the reader, are not the intended recipient you are hereby notified
> >> that any dissemination, distribution or copying of this communication
> >> is strictly prohibited. Do not read the email but instead reply to
> >> the sender and destroy the message and any attachments. Thank you.
> >> _______________________________________________
> >> Histonet mailing list
> >> Histonet <@t> lists.utsouthwestern.edu
> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> >
> > ------------------------------
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> > End of Histonet Digest, Vol 134, Issue 28
> > *****************************************
>
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