[Histonet] General Tissue Processing

[ian bernard]

Fixative is best or 10 % NBF

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hernandez,
Jesus Willibaldo
Sent: Friday, January 16, 2015 7:54 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] General Tissue Processing

I had a quick question about processing bone samples. Since we do not have
an automatic tissue processor, what are the recommended solutions that I can
leave my samples overnight in without ruining them. Last time I processed
tissue, I left the samples overnight in 80%EtOH and then in 100%EtOH. Is
this acceptable or will this affect the overall tissue morphology? Thank
you.

Regards,

Jesse H.
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