[Histonet] MMA Troubleshooting for Undecalcified Bone

[Hernandez, Jesus Willibaldo]

This is intended for Bone Histology samples embedded in MMA. I had two main questions.


1.       After mounting my tissue sections (5-10microns) with Haupts adhesive, I noticed that the bone within the section was appearing opaque/darkened on the microscope? I will add that the morphology of the bone structure is not present. The bone still stains, but the detail is missing. I am thinking that I might of over dehydrated during processing or my microtome blade is dull. Any suggestions about this problem would be appreciated.



2.       Anyone have a Hematoxylin & Eosin Staining protocol for undecalcified MMA embedded bone? I do have a protocol, but not sure if I was missing a couple of steps for proper staining.



Thank you all.

Regards,

Jesse Hernandez