[Histonet] marking small samples

[Morken, Timothy]

Last year I found a paper to the effect that eosin may interfere with PCR analysis. Our molecular group did an internal study and did not find any significant issues.

But I just found another paper stating no interference:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3383644/

Am J Clin Pathol. Jul 2012; 138(1): 122-129.
No Evidence for Interference of Hematoxylin and Eosin (HE) Staining in DNA Testing: Utility of DNA Extraction from HE-Stained Archival Tissue Sections
Teppei Morikawa, MD



im Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

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-----Original Message-----
From: Melissa Burns [mailto:mburns <@t> atlanticurologyclinics.com] 
Sent: Wednesday, January 14, 2015 2:57 PM
To: Morken, Timothy
Cc: Yves Heremans; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] marking small samples

I've been told Eosin affects a lot of molecular testing. We used to use it in our process but discontinued to not interfere with future testing.  

Sent from my iPhone

> On Jan 14, 2015, at 5:31 PM, "Morken, Timothy" <Timothy.Morken <@t> ucsf.edu> wrote:
> 
> We use eosin the processor alcohol. Also we use blue-tinted paraffin 
> which helps with contrast on small bx
> 
> Tim Morken
> Supervisor, Histology, Electron Microscopy and Neuromuscular Special 
> Studies UC San Francisco Medical Center San Francisco, CA
> 
> CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu 
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Yves 
> Heremans
> Sent: Wednesday, January 14, 2015 2:03 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] marking small samples
> 
> Dear Histonetters,
> 
> Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ?
> Preferably something that would not interfere with subsequent antibody staining.
> 
> Yves
> 
> 
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