[Histonet] p16 Antibody

[Martha Ward-Pathology]

We dilute ours 1:5 from the predilute and run it on the Bond 3.   It works very well.

 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mward <@t> wakehealth.edu  
 
 



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy
Sent: Wednesday, January 14, 2015 10:15 AM
To: 'Sarah.Dysart <@t> stdavids.com'; LSebree <@t> uwhealth.org; veronique.barres <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] p16 Antibody

We use the Ventana (INK4a) clone as well. What dilution do you use?

Brandy Burnett
Histotechnoligist, QIHC(ASCP)
CCH Pathology/Histology
508-862-5267
bburnett <@t> capecodhealth.org


        Expert physicians. Quality hospitals. Superior care. 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sarah.Dysart <@t> stdavids.com
Sent: Wednesday, January 14, 2015 10:01 AM
To: LSebree <@t> uwhealth.org; veronique.barres <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] p16 Antibody

Ventana unfortunately has the market on the "good" clone...you can dilute it out pretty far (it comes pre-diluted) and still get good results however...


Sarah Dysart  BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor North Austin Medical Center
12221 North Mopac Expressway
Austin, TX  78758
512-901-1220 (lab)
512-901-6659 (office)



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Wednesday, January 14, 2015 8:41 AM
To: 'Véronique Barrès'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] p16 Antibody

We use one from BD, Veronique, with good results. 

Linda A. Sebree
University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave. 
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Véronique Barrès
Sent: Wednesday, January 14, 2015 8:06 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] p16 Antibody

Hi Histonetters!

I am working in a research lab and we sometimes need to do p16 staining, but our pathologist told us that the antibody we are using right now is not good. I'd like to know which antibody you're using in your labs?

Thanks for your help and happy Wednesday!
Véronique
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