And other crazy stuff. RE: [Histonet] cutting honey bees

[Jennifer MacDonald]

Termites for a science fair project.
anchovy from a pizza to pull a joke on a pathologist
chicken bone from dim sum chicken feet, again to pull one over on a 
pathologist.  Not decalcified, embedded in resin.



From:   "Morken, Timothy" <Timothy.Morken <@t> ucsf.edu>
To:     Patsy Ruegg <pruegghm <@t> hotmail.com>, Roberta Horner <rjr6 <@t> psu.edu>, 
"Douglas Gregg" <classicdoc <@t> gmail.com>, "Histonet <@t> Lists. Edu" 
<histonet <@t> lists.utsouthwestern.edu>
Date:   01/08/2015 12:00 PM
Subject:        And other crazy stuff.  RE: [Histonet] cutting honey bees
Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu



You crazy research people...OK, so what is the craziest thing you ever had 
to cut, or were asked to cut?

For me, not too bad, but embedding for EM and sectioning a single oocyte 
that was nearly microscopic. I'll just say it took a LOT of thick sections 
too face down to it without actually cutting through it.


Open the floodgates....

Tim Morken

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [
mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Tuesday, January 06, 2015 11:13 AM
To: Roberta Horner; Douglas Gregg; Histonet <@t> Lists. Edu
Subject: RE: [Histonet] cutting honey bees

for the whole bee I probably would process and embed it in glycol 
methacrylate (gma) it is much harder and would give better sections, we 
have done zebra fish and several other harder tissues including calcified 
bone in GMA.

Cheers,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm <@t> hotmail.com



> From: rjr6 <@t> psu.edu
> To: classicdoc <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
> Date: Sat, 3 Jan 2015 23:15:33 +0000
> Subject: RE: [Histonet] cutting honey bees
> CC: 
> 
> I sectioned and stained honey bee and yellow jacket stingers years ago. 
They wanted to show the difference between the stingers.  I wasn't sure 
what to do so I processed and handled like everything else.  I was able to 
get some good sections.  I put 6 stingers in each block and cut several 
sections figuring there should be at least one good stinger in each block 
and it worked.
> Roberta Horner
> Penn State University
> Animal Diagnostic Lab
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu 
> [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Douglas Gregg 
> [classicdoc <@t> gmail.com]
> Sent: Saturday, January 03, 2015 6:08 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] cutting honey bees
> 
> Has anyone had experience embedding and cutting honey bees. I am sure 
> there are some issues with the harder exoskeleton. Would that have to 
> be dissected away first. I am considering helping a student with a 
> science fair project on bees.
> 
> Douglas Gregg
> Veterianary pathologist
> 
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