[Histonet] X-gal staining

[koellingr <@t> comcast.net]

Kristopher, 
beta-gal is an enzyme and as far as I know is rendered inert after FFPE.  I did do fresh skin in X-gal to target, as you would with any whole mount specimen, THEN FFPE and cut sections to it to see the signal.  If that is not an option, go after it with an anti-beta galactosidase antibody (polyclonal-like Pierce I used-have zero relationship to them) from several places.  Make sure the immunogen is a full, length protein that produced the antibody, and it will go much easier. 
  
Ray 
  
Raymond Koelling 
Seattle, WA area 

----- Original Message -----

From: "Kristopher Kalleberg" <Kristopher.Kalleberg <@t> unilever.com> 
To: histonet-request <@t> lists.utsouthwestern.edu, histonet <@t> lists.utsouthwestern.edu 
Sent: Thursday, January 8, 2015 9:53:38 AM 
Subject: [Histonet] X-gal staining 

Hello All, 

Does anyone know of a commercially available X-gal stain that would work on formalin fixed paraffin embedded skin samples in order to detect beta galactosidase.  Thank you in advance. 

Kris 
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