And other crazy stuff. RE: [Histonet] cutting honey bees

Wed Jan 7 08:14:26 CST 2015

You guys are so cool!! Thanks for sharing your stories - I love reading
all of the cool stuff histotechs are doing out in the world!

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones <@t> metropath.com

On 1/6/15, 5:38 PM, "Caroline Miller" <mills <@t> 3scan.com> wrote:

>When I worked in a research core (which was only last week, but I just
>changed jobs). I have processed and cut (with varying degrees of success)
>fake meat samples for a company. They were mainly made of grains and
>mushed veggies. 
>
>The problem was the samples were not consistent and there was no room
>(time or money)  for honing the protocol for each of the many samples he
>sent me, so he got what I could cut. He always seemed pleased, but I
>couldn't see much in the samples. He was very secretive about it all, so
>I could never quite understand what they were doing t all for!!!
>
>C
>
>Sent from my iPhone
>
>> On Jan 6, 2015, at 12:54 PM, Roberta Horner <rjr6 <@t> psu.edu> wrote:
>> 
>> The oddest things I cut were the honey bee and yellow jacket stingers.
>>I've done plant stamen, reptiles, fish and I believe another insect.  I
>>usually tell the students that are working on a research project to give
>>me a sample they don't care about so I can see if I can do what they
>>want.
>> 
>> But I had oddities that I didn't have to section like during hunting
>>season a hunter killed a deer and there was a mass on the trachea that
>>he wanted tested to make sure the deer was okay to eat.  I got the
>>sample and when I tried to gross it I found a very hard shiny silver
>>object.  I told the pathologist whose case it was that the mass was from
>>a bullet did he still want histo done. No.
>> 
>> The other interesting one was the egg shell.
>> The conversation went something like this.
>> Pathologist:  Can you section this egg shell
>> Me: No it's too hard.
>> P: Can't you decal it
>> M: That's not going to work.
>> P: Did you try.
>> M: No
>> P: Don't you think you should try first.
>> M: Okay fine but it is no going to work.
>> 
>> Put a piece of eggshell (made of calcium) into some decal solution
>>(that removes calcium) and watch the egg shell bubble and disappear.  I
>>did get to tell the pathologist "I told you so"
>> 
>> Roberta Horner
>> Penn State University
>> Animal Diagnostic Lab
>> 
>> -----Original Message-----
>> From: Morken, Timothy [mailto:Timothy.Morken <@t> ucsf.edu]
>> Sent: Tuesday, January 06, 2015 2:24 PM
>> To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet <@t> Lists. Edu
>> Subject: And other crazy stuff. RE: [Histonet] cutting honey bees
>> 
>> You crazy research people...OK, so what is the craziest thing you ever
>>had to cut, or were asked to cut?
>> 
>> For me, not too bad, but embedding for EM and sectioning a single
>>oocyte that was nearly microscopic. I'll just say it took a LOT of thick
>>sections too face down to it without actually cutting through it.
>> 
>> 
>> Open the floodgates....
>> 
>> Tim Morken
>> 
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy
>>Ruegg
>> Sent: Tuesday, January 06, 2015 11:13 AM
>> To: Roberta Horner; Douglas Gregg; Histonet <@t> Lists. Edu
>> Subject: RE: [Histonet] cutting honey bees
>> 
>> for the whole bee I probably would process and embed it in glycol
>>methacrylate (gma) it is much harder and would give better sections, we
>>have done zebra fish and several other harder tissues including
>>calcified bone in GMA.
>> 
>> Cheers,
>> Patsy
>> 
>> Patsy Ruegg, HT(ASCP)QIHC
>> Ruegg IHC Consulting
>> 40864 E Arkansas Ave
>> Bennett, CO 80102
>> H 303-644-4538
>> C 720-281-5406
>> pruegghm <@t> hotmail.com
>> 
>> 
>> 
>>> From: rjr6 <@t> psu.edu
>>> To: classicdoc <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
>>> Date: Sat, 3 Jan 2015 23:15:33 +0000
>>> Subject: RE: [Histonet] cutting honey bees
>>> CC: 
>>> 
>>> I sectioned and stained honey bee and yellow jacket stingers years
>>>ago.  They wanted to show the difference between the stingers.  I
>>>wasn't sure what to do so I processed and handled like everything else.
>>> I was able to get some good sections.  I put 6 stingers in each block
>>>and cut several sections figuring there should be at least one good
>>>stinger in each block and it worked.
>>> Roberta Horner
>>> Penn State University
>>> Animal Diagnostic Lab
>>> ________________________________________
>>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>>> [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Douglas Gregg
>>> [classicdoc <@t> gmail.com]
>>> Sent: Saturday, January 03, 2015 6:08 PM
>>> To: histonet <@t> lists.utsouthwestern.edu
>>> Subject: [Histonet] cutting honey bees
>>> 
>>> Has anyone had experience embedding and cutting honey bees. I am sure
>>> there are some issues with the harder exoskeleton. Would that have to
>>> be dissected away first. I am considering helping a student with a
>>> science fair project on bees.
>>> 
>>> Douglas Gregg
>>> Veterianary pathologist
>>> 
>>> _______________________________________________
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>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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