[Histonet] PASD muscle stains

[koellingr <@t> comcast.net]

Tiffany, 
Have used 10%NBF on muscles but also alcoholic fixatives -alcoholic formalin or absolute- just always preferred 10%NBF since it gave the morphology and counterstaining I wanted.  diastase in a 6.0pH buffer (don't heat above 40 degrees if trying to speed up heating-kill the diastase) and always stayed away from di water on frozen sections.  di water too variable and fickle. 
  
Ray Koelling 
Lake Forest Park 

----- Original Message -----

From: "Tiffany Passaro" <tpassaro <@t> cellnetix.com> 
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Monday, January 5, 2015 3:22:32 PM 
Subject: [Histonet] PASD muscle stains 

Greetings, 


                I am looking for fixatives that others are using in their labs for the PASD stain on fresh frozen muscle tissue. Currently we are fixing in 10% NBF. Thanks in advance for any info on this. 

Tiffany 
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