[Histonet] HELP! Need some old fashioned histology advice

[Jay Lundgren]

I can help with the old fashioned advice:


   - 1 scant teaspoon simple syrup
   - 2 dashes Angostura Bitters, plus more to taste
   - 1 half dollar–sized slice orange peel, including pith
   - 2 ounces good-quality rye or bourbon
   - 1 maraschino cherry

     As for the Histology, is there any reason you cannot mount the
sections onto glass slides?  When I was working at Genentech they were
cutting frozen sections through whole rabbits and mounting the sections on
(giant) glass slides.  I think that rolling the tissue up, inserting it,
and then removing it from a glass tube would destroy the tissue.

                                      Sincerely,

                                            Jay A. Lundgren, M.S., HTL
(ASCP)

On Sat, Jan 3, 2015 at 8:01 PM, Maria Mejia <mbmphoto <@t> gmail.com> wrote:

> First, the very best of holidays to everyone.
>
> Now for the histology part.   Our lab's focus is on the early stages of
> Alzheimer's Disease in the Brainstem
> using celloidin processing & embedding for IHC staining.  This year, our
> lab will be receiving 6 post-mortem
> whole human brains (1 every other month).  After fixation, processing &
> celloidin embedding, the whole brain
> will be serially cut at 100um thick.  Each brain section will be 5 inches
> x 4.5 inches in size.
>
> I will given 250 of these whole brain sections to stain for tau
> IHC...that's 1500 whole brain sections/year!!!
> 1) Does anyone have experience doing manual IHC staining of large
> free-floating brain sections?
> 2) What type of staining tools, dishes or other essential equipment can
> anyone recommend?
> 3) What's the most efficient way to stain 250 sections for batch IHC
> staining - such as transferring batch
> sections (maybe 5-10) from reagent to reagent?
> 4) What type of batch apparatus to use?
>
> As for the antibody & ABC steps, I was thinking of placing each section
> inside a large glass cigar tube
> (yep, people use large glass tubes with fitted cap to store cigars), with
> 5ml of antibody or ABC reagent & gently agitate on
> a shaker/rotator at room temp during the incubation.  Does anyone have
> ideas on this?
>
> Please, any ideas, suggestions or recommendation anyone can provide will
> be most greatly appreciated.
>
> Best regards
> Maria Mejia
> UCSF
> Department of Neurology
> San Francisco, CA
>
>
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