[Histonet] RE: Ventana Ultra CD10

[Garrison, Becky]

For Ventana CD10, (SP67), we use the extended CC1 (92 minutes), antibody for 36 minutes and amplify on the Ultra.

Becky Garrison
Pathology Supervisor
Shands Jacksonville
Jacksonville, FL 32209
904-244-6237, phone
904-244-4290, fax
904-393-3194, pager
 
  
 
 
 
 
 
 
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of O'neil, Beth
Sent: Thursday, February 26, 2015 4:22 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ventana Ultra CD10

We are in the last stages of contract negotiations for purchasing two Ventana Benchmark Ultras.  During this time period we have been optimizing our current inventory of approximately 110 antibodies and validating before our current instrumentation is removed within the next two weeks.  My Ventana application specialist is unable to successfully optimize CD10 (Ventana clone SP67) on the Ventana Benchmark Ultra.  He had me send slides to their applications/troubleshooting lab but they told us they won't start working on it until next week and then it would take about a week for them to try and optimize it.  We are in an urgent rush to get this antibody optimized and validated within the next two weeks since it is heavily requested by our Hemepaths.  Would anyone be willing to share their Ultra protocols with me?  Has anyone had similar experiences with Ventana being unsuccessful and having to send their slides to their applications lab for work up?  Thank you for your help.

Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
oneilb <@t> wvuhealthcare.com<mailto:oneilb <@t> wvuhealthcare.com>
Histology Supervisor, Technical Specialist
Lab:  304 - 293 - 6014
Office:  304 - 293 - 7629



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