[Histonet] RE: Retic Kit Problems

[Jeffrey Robinson]

Yes, Blake, the Benchmark Special Stainer does a very poor job on retics, especially bone marrow retics.  We got one in December, 2013, and we do not use it for retics (I still have an old Nexes).  I had a Ventana guy here multiple times as they thought it was a contamination problem.  He decontaminated it several times and they changed some interior filters which helped some.  The problem is the heat (or lack of), not the kit.  The old Nexes would heat the entire chamber with great results.  The new one has the individual heating pads but the current protocol does not give it enough heat for the retic.  Ventana says a software upgrade is coming out in the second quarter of this year that will hopefully fix the problem.  Keep your fingers crossed!

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Blake Taylor
Sent: Thursday, February 12, 2015 10:06 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Retic Kit Problems

Anyone using the Ventana benchmark special stainers encountering inconsistent staining on your Retics? Same Run one will look great the other not staining properly.  Re run it and no issues.  We are shaking the kit well before each use and have increased how often we decon.  Any thoughts?

Blake Taylor
Surgical Pathology Supervisor
Lexington Medical Center
803-936-8214
bcdukes <@t> lexhealth.org<mailto:bcdukes <@t> lexhealth.org>

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